Deinococcus radiodurans is a highly UV and radio resistant bacterium. The addition of Mn2+ could induce an Mn-CD effect in this bacterium. In this study, we used two-dimensional polyacrylamide gel electrophoresis to compare and analyze the expressed-proteins under various growth conditions, such as temperature and the presence of Mn2+ or not. The results showed that Mn2+ could affect the similarity proteins expression. As the time of Mn-CD effect elapsed longer, the similarity of the proteins from different growth phages became lower. This indicated that Mn2+ indeed could induce or repress the gene expression. From the 2-D gel analysis, there were fourteen proteins had been induced or overexpressed. Five of them were the proteins with the functions for the synthesis and decomposition of proteins and DNA, others were ATP-binding cassette¡]ABC¡^transporter¡Bsuperoxide dismutase［Mn］, and the rest five were the hypothetical proteins with unclear function. In addition, this study also found that the cultivation temperatures caused conformational and physiological modification of the cell. The addition of Mn2+ could enhance the viability of the bacterium at higher temperature.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0827104-142541 |
| Date | 27 August 2004 |
| Creators | Huang, Kwun-lun |
| Contributors | Chi-hsin Hsu, Ting-fe Wu, Jong-kang Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827104-142541 |
| Rights | campus_withheld, Copyright information available at source archive |
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