This study evaluated the ability of photodynamic therapy (PDT), chitosan nanoparticles
(CSnp), or their combination, to inhibit bacterial collagenase-mediated degradation of
collagen. Rat type 1 fibrillar collagen matrices were untreated or treated with 2.5%
glutaraldehyde (GD), 2.5% GD followed by 1% CSnp, 1% CSnp, PDT, or 1% CSnp
followed by PDT. Samples, except untreated controls, were exposed to Clostridium
histolyticum collagenase. The soluble digestion products were
assessed by hydroxyproline assay and the remaining adherent collagen was quantified by
picrosirius red (PSR) staining. Collagen treated with CSnp, PDT, or a combination of
CSnp and PDT, exhibited less degradation than controls. The abundance of posttreatment
residual collagen correlated with the extent of degradation. Fourier transform
infrared (FTIR) spectroscopy analysis showed that PDT treatment enhanced collagen
cross-linking. Immunoblotting of sedimented CSnp indicated that CSnp and collagenase
bound with low affinity. However, CSnp-bound collagenase showed a significant
reduction in collagenolytic activity compared with controls.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/43293 |
| Date | 09 December 2013 |
| Creators | Persadmehr, Anousheh |
| Contributors | Kishen, Anil |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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