A rodent model was developed to assess the osteogenic potential of continuous capacitive electrical stimulation on bone healing around titanium (Ti) implants. Adult male rats were fitted subcutaneously with electrical leads, that were placed around Ti implants inserted in the tibia. One electrical lead was attached directly to the extraosseous component of the implant, and the other was anchored to the adjacent subcutaneous tissues. Bone healing around the Ti implant was assessed histologically after 2, 3, 4, 5, 6, 7, 14 and 21 days of continuous capacitive electrical stimulation as compared to controls and sacrificed immediately. Other groups of animals were given 7 days of continuous capacitive electrical stimulation but sacrificed at day 14 or 21 post-implantation. Histomorphometric analysis of the bone-Ti interface revealed that animals given 5--7 days of continuous capacitive electrical stimulation demonstrated more bone at the surface of the Ti implant than in controls. Radioautographic analyses of bone formation was carried out in rats injected with tritiated (3H)-proline 4 days prior to sacrifice at days 7, 14 and 21. These results showed increased 3H-proline deposition at day 7, corresponding to increased bone formation as shown by morphometric findings. Biomechanical 'pull-out' tests demonstrated that the force required to remove the Ti implants from the tibiae was significantly greater in electrically stimulated animals compared to controls at day 7. Bone densitometric measurements revealed a statistically significant increase in bone density in the electrically-stimulated animals by days 5--7 compared to controls. In summary, an animal model was developed to evaluate the effects of continuous capacitive electrical stimulation on bone healing around Ti implants placed in tibiae of rats. Significant bone growth was demonstrated by days 5--7 postimplantation as in all parameters tested. These findings suggest that continuous capacitive electrical stimula
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.37858 |
| Date | January 2001 |
| Creators | Abd-Ul-Salam, Hani. |
| Contributors | Gornitsky, M. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Faculty of Dentistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001838099, proquestno: NQ75598, Theses scanned by UMI/ProQuest. |
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