The detection and identification of particles within fluid samples is key in the prevention of the spreading of disease. This has created a growing need for devices able to successfully separate and identify multiple particles for this purpose while operating at a high enough throughput to be applicable in the field. A well investigated method of manipulating particles in this way is Dielectrophoresis (DEP), which is the use of varied electric fields gradients to generate a force on small particles. The strength of DEP depends of the properties of the particle medium, the signal generating the electric field, and the properties of the particles themselves. This method and its interaction with all small particles, including biological particles such as blood and cancer cells, has allowed devices utilizing this idea to be investigated for various biological purposes. This thesis investigates methods to increase the throughput of these types of devices in order to increase their ability to process large amounts of samples in reasonable amounts of time. This is done in primarily two methods. One approach uses the application of chromatographic methods to DEP devices to separate particles by altering their individual transit time through a device, allowing identification during constant flow. Another method is through mass parallel channels which each individually operate as a standard DEP particle trapping device. This allows for the summation of the maximum flow through the device due to its design layout. / Master of Science / Micrometer scale devices are popular for the identification, separation, and characterization of micron scale particles. This includes uses in biological fields for the manipulation of particles such as blood cells, cancer cells, and bacteria. A common method of manipulating these particles is Dielectrophoresis, a force that causes particles to be repelled or attracted to geometric designs within the device generated by an applied electric field. The strength and direction of this force on the particles is dependent on the properties of the electrical signal applied to the device, the physical properties of the particles, such as size and shape, and the properties of the medium the particles are suspended in within the device. Biological devices utilizing this force have been tested before, allowing for particles to be separated out of mixed particle solutions. Most of these devices operate by moving through very little material at one time, somewhere in the microliter per hour range. This thesis explores attempts to increase the rate at which samples can be processed by these devices in multiple ways. Chapter 2 explores methods of DEP by applying Chromatography principles, which is to constantly move samples through the device at a high rate and slow the target particles, so they exit the device at a different time than other particles. Chapter 3 investigates increasing device throughput by replicating a standard DEP channel multiple times on one device so that several may operate all at once.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/108241 |
| Date | 18 August 2020 |
| Creators | Ervin, Allen Dale |
| Contributors | Electrical Engineering, Agah, Masoud, Baumann, William T., Jia, Xiaoting |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Detected Language | English |
| Type | Thesis |
| Format | ETD, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
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