by King Lai Wong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 86-93). / Title Page --- p.i / Table of Contents --- p.ii / List of Tables --- p.iv / List of Figures --- p.v / Abbreviations --- p.viii / Acknowledgements --- p.ix / Abstract --- p.x / Chapter CHAPTER ONE --- INTRODUCTION / Chapter 1.1. --- Mass Spectrometry for Macromolecular Analysis --- p.1 / Chapter 1.2. --- Laser Desorption --- p.3 / Chapter 1.3. --- Development of Matrix-assisted Laser Desorption/Ionization (MALDI) --- p.4 / Chapter 1.4. --- Matrix-assisted Laser Desorption/Ionization --- p.5 / Chapter 1.4.1. --- Laser --- p.6 / Chapter 1.4.2. --- Matrix Selection --- p.6 / Chapter 1.4.3. --- Sample Preparation Methodology --- p.7 / Chapter 1.4.4. --- Ion Formation Process(es) --- p.7 / Chapter 1.4.4.1. --- Desorption --- p.8 / Chapter 1.4.4.2. --- Ionization --- p.9 / Chapter 1.5. --- Time-of-Flight Mass Spectrometry --- p.12 / Chapter 1.6. --- Outline of the Present Work --- p.16 / Chapter CHAPTER TWO --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1. --- Instrumentation --- p.17 / Chapter 2.1.1. --- Laser System --- p.17 / Chapter 2.1.2. --- Ion Source --- p.19 / Chapter 2.1.3. --- Reflector --- p.20 / Chapter 2.1.4. --- Detector --- p.20 / Chapter 2.2. --- Experimental --- p.21 / Chapter 2.2.1. --- Synthesis of nitroanthracene-d9 --- p.21 / Chapter 2.2.2. --- Sample Preparation --- p.22 / Chapter CHAPTER THREE --- STUDIES OF THE EFFECTS OF SOLUTION pH / Chapter 3.1. --- Introduction --- p.25 / Chapter 3.2. --- Sample Preparation --- p.26 / Chapter 3.3. --- Results and Discussion --- p.28 / Chapter 3.3.1. --- Effect of Bronsted Base (NaOH) --- p.28 / Chapter 3.3.2. --- Effect of Lewis Base (Imidazole) --- p.33 / Chapter 3.3.3. --- Effect of Salt Concentration --- p.40 / Chapter 3.4. --- Conclusions --- p.44 / Chapter CHAPTER FOUR --- PROTON SOURCES FOR ION GENERATION IN MALDI-MS / Chapter 4.1. --- Introduction --- p.46 / Chapter 4.2. --- Sample Preparation --- p.47 / Chapter 4.3. --- Results and Discussion --- p.49 / Chapter 4.4. --- Conclusions --- p.55 / Chapter CHAPTER FIVE --- CATIONIZATION PROCESSES IN MALDI-MS : ATTACHMENT OF DIVALENT AND TRIVALENT METAL IONS / Chapter 5.1. --- Introduction --- p.57 / Chapter 5.2. --- Sample Preparation --- p.58 / Chapter 5.3. --- Results and Discussion --- p.60 / Chapter 5.3.1. --- Protonation versus Cationization --- p.60 / Chapter 5.3.2. --- Attachment of Divalent and Trivalent Metal Ions --- p.63 / Chapter 5.4. --- Conclusions --- p.80 / Chapter CHAPTER SIX --- CONCLUDING REMARKS --- p.82 / REFERENCES --- p.86
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322682 |
| Date | January 1997 |
| Contributors | Wong, King Lai., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xi, 93 leaves : ill ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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