This study explores the application of two methods of spectroscopy; Near Infrared
spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches
for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study
were twofold: The first was to determine if cortisol can be identified within the NIR
and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second
objective was to determine the capability of NIR and FTIR to predict FGM
concentrations obtained using standard laboratory methods. Fecal glucocorticoid
concentrations measured by Enzyme Immunoassay were used as the reference data of
partial least square (PLS) regression of fecal extract NIR spectra and FTIR spectra. Low
accuracies (NIR: R2 = 0.152; FTIR: R2 = 0.199) were obtained from regression models
using data from both methods. Though this study did not successfully demonstrate the feasibility of using NIR and FTIR to qualify and quantify FGMs, it is likely not a
reflection of the capabilities of the technology, but rather of appropriate sample types and
preparation methods. / Includes bibliography. / Thesis (M.A.)--Florida Atlantic University, 2016. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_33697 |
| Contributors | Litman, Benni (author), Broadfield, Douglas C. (Thesis advisor), Harris, Michael S. (Thesis advisor), Florida Atlantic University (Degree grantor), Dorothy F. Schmidt College of Arts and Letters, Department of Anthropology |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 81 p., application/pdf |
| Rights | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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