The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc277940 |
| Date | 08 1900 |
| Creators | Al-Khatib, Haifa Yousef |
| Contributors | Shanley, Mark Stephen, O'Donovan, Gerard A., Gracy, Robert W. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 87 leaves : ill., Text |
| Rights | Public, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Al-Khatib, Haifa Yousef |
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