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Previous issue date: 2014-09-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A obesidade ? considerada mundialmente como sendo uma doen?a grave, ocupando o quinto lugar em ?ndice de ?bitos. Em 2009 e 2010 foi lan?ado o medicamento denominado Victoza? com o objetivo de reduzir peso em indiv?duos portadores de diabetes mellitustipo 2, evitando assim o surgimento de outras doen?as associadas. O agravante ? que indiv?duos obesos n?o diab?ticos est?o fazendo uso desta subst?ncia em busca do emagrecimento, mesmo quando n?o autorizado pela ANVISA. Assim o objetivo desta pesquisa ?avaliar o efeito do Victoza? nos tecidos muscular e adiposo e nos par?metros bioqu?micos em camundongos Swiss obesos n?o diab?ticos. O estudo foi aprovado pela Comiss?o de ?tica no Uso de Animais - CEUA (Protocolo n?003/2014), onde foram utilizados 74 animais (camundongos Swiss). Na fase inicial deste estudo, realizou-se um estudo piloto (n = 10) divididos em grupo controle (CON) (n = 5) e grupo cafeteria (CAF) (n = 5) para a padroniza??o do melhor card?pio para indu??o a obesidade atrav?s de uma dieta de cafeteria. Nos animais do grupo CAF a adiposidade intra-abdominal (0,74 ? 0,05 mg) foi tida como par?metro para visualiza??o da efic?cia do experimento. Posteriormente foi realizado o estudo base para esta pesquisa onde foram utilizados animais (n = 64) divididos em 8 grupos. O grupo dos obesos (CAF) foram divididos da seguinte forma: obesidade + exerc?cio + Victoza? (OEV) (n = 8), obesidade + exerc?cio + salina (OES) (n = 8), obesidade + Victoza? (OV) (n = 8) e obesidade + salina (OS) (n = 8). O grupo dos n?o obesos (CON) foram divididos em: exerc?cio + Victoza? (EV) (n = 8), exerc?cio + salina (ES) (n = 8), Victoza? (V) (n = 8) e solu??o salina (SS) (n = 8). No andamento do estudo os animais passaram pelos seguintes processos: indu??o a obesidade atrav?s da dieta de cafeteria, seguidos do protocolo de exerc?cio atrav?s da nata??o aer?bia, tratamento com a subst?ncia teste via intraperitoneal (200 ?g/mL/kg). Ap?s, os animais foram eutanasiados sendo avaliados: a massa dos tecidos muscular e adiposo e os par?metros bioqu?micos. Verificou-se que o Victoza? reduziu a massa de tecido adiposo (0,32 ? 0,05 mg), quando comparados ao grupo salina (0,53 ? 0,07 mg). Verificou-se tamb?m que n?o houve altera??es no tecido muscular do grupo tratado com Victoza? (5,53 ? 0,05 g) quando comparado ao grupo salina (1,33 ? 0,06 g). Com rela??o aos estudos bioqu?micos ficou evidenciado que houve altera??es nestes par?metros, no entanto um importante marcador que ? a glicose, foi alterado mas de maneira a n?o tornar os animais diab?ticos. Assim, conclui-se a import?ncia da pratica da atividade f?sica unida ? utiliza??o do Victoza? para um melhor resultado em termos de ganho de massa magra e perda de tecido adiposo gerando assim um resultado satisfat?rio na busca da qualidade de vida e preven??o de doen?as. / Obesity is a chronic metabolic disease characterized by adipose tissue formation excess leading to an increase in body fat mass, of multifactorial origin, produced mainly by poor eating habits combined with a sedentary lifestyle. Data consider obesity as a serious disease that affects the world's population, ranking fifth in death rates. Faced with this situation, individuals seek, increasingly, means to lose weight with less physical effort and food. In 2009 and 2010 the drug liraglutide was lauched in order to reduce weight in individuals with diabetes mellitus type 2, thus avoiding the emergence of other diseases. The aggravating factor is that obese nondiabetic individuals are making use of this substance, even if its use is not authorized by ANVISA (Brazilian Health Surveillance Agency). Thus the objective of this research is to evaluate the effect of liraglutide for muscle or fat tissues and biochemical parameters in Swiss mice submitted to cafeteria diet and physical activity. The study was approved by the Ethics Committee on Animal Use - CEUA (n?003 Protocol / 2014). For this study 74 animals (Swiss mice) were used, divided as follows: in the initial phase of this study, we carried out a pilot study (n = 10) divided into a control group (PCON) (n = 5) and cafeteria group (PCAF) (n = 5), in order to evaluate a cafeteria diet which was both attractive to the animals and that could provide an increase in adipose tissue. After the induction of the diet, animals were euthanized and as a result, the animals in the PCAF group showed an intra-abdominal adiposity 0.74 ? 0.05 g, taken as the parameter for increasing fat in animals. Subsequently the study base was conducted for this research where animals were used (n = 64) divided into 2 groups: the Cafeteria Study Base Group (EBCAF) divided as follows: cafeteria + exercise + liraglutide (CEL) (n = 8), cafeteria + exercise + saline (CES) (n = 8), cafeteria + liraglutide (CL) (n = 8) and cafeteria + saline (CS) (n = 8). The Chow Study Base group (EBR) was divided into: exercise + liraglutide (EL) (n = 8), exercise + saline + (ES) (n = 8), liraglutide (L) (n = 8) and saline solution (SS) (n = 8). All animals went through the submission process to the cafeteria diet, followed by exercise protocol through swimming and treatment with the test substance intraperitoneally (200 mg / mL / kg). After the treatments, the animals were euthanized and had the following parameters evaluated: the muscle tissue mass, adipose tissue mass and biochemical parameters. It was observed that the processing done with the exercise-associated liraglutide reduced adipose tissue mass significantly (0.32 ? 0.05 g) compared to the saline group (0.53 ? 0.07 g). There were no changes in the muscle tissue of the group which was treated and exercised (1.39 ? 0.03 g) compared to the saline group (1.33 ? 0.03 g). Regarding biochemical parameters it was evident that there were changes in these parameters. Interesting to note that, although blood glucose values have been changed, the animals did not become diabetic. Thus, it appears that physical activity together with liraglutide is eficcient to the loss of intraabdominal adipose tissue and the maintenance of lean body mass thereby generating a satisfactory result in the pursuit of quality of life and disease prevention.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20825 |
| Date | 22 September 2014 |
| Creators | Silva, Cybelle de Arruda Navarro |
| Contributors | 70155780425, http://lattes.cnpq.br/4311626091295357, Costa, Eduardo Caldas, 05304117417, http://lattes.cnpq.br/1216441676725839, Fayh, Ana Paula Trussardi, 95978887004, http://lattes.cnpq.br/0049770583345803, Silva, Bagn?lia Ara?jo da, 48668443453, http://lattes.cnpq.br/2569484428391315, Lemos, Telma Maria Ara?jo Moura |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM EDUCA??O F?SICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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