Abstract
Ultrasonic technique is widely used in medical application and food industry; however, much work has focused on harmful biological effects of tissues and cells by ultrasound exposure; only little information is mentioned about the beneficial biological effects of ultrasound. Thus, the objective of this thesis is to observe the beneficial biological activities of Paramecium induced by ultrasound exposure.
Since the structure or biophysical will stimulated into the interaction between ultrasound and living matter. When multi-cell creature is exposed by ultrasound, this reaction will lead the biological effect becomes complex. Therefore, a single cell creature is chosen to understand the beneficial biological effects induced by ultrasound exposure. The oscillation of the monad in response to the ultrasound radiation is simulated using Rayleigh-Plesset¡¦s bubble activation theory. The resonance frequency of the unicellular creature is then calculated. The diffuse field theory of Sabine is used to create a uniform sound field for the radiation experiment. The images obtained from a microscope can be analyzed and recorded by a personal computer. The number of cells was counted in the haemacytometer after irradiation.
The calculated resonant frequency range of the Paramecium shape is 0.2~0.27 MHz. The relative growth rate of the Paramecium suspensions exposed to ultrasound was about 20% slower than that of unexposed sample. It was found that the phenomenon of inhibition and destruction appeared during irradiation. Also, the growth curve is retarded during the period. The resonance frequency of the Paramecium vacuole is 0.5~1.09 MHz. The maximum relative growth rate was increasing 18% with 1MHz irradiation.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0711101-214058 |
| Date | 11 July 2001 |
| Creators | Chen, Ming-Kai |
| Contributors | Shiuh-Kuang Yang, Rui-Chang Wei, Ruyen Ro |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711101-214058 |
| Rights | unrestricted, Copyright information available at source archive |
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