New methodology has been utilized to provoke or increase targeted metabolic pathways in microbes. The low hanging fruit of natural products has been discovered over the last 50 years. To continue finding new metabolites to be used as possible drug candidates, methodology development such as those proposed herein are necessary. This methodology uses extracts from known pathogenic bacteria to elicit production of latent biosynthetic pathways from environmental bacterial isolates that may be active against the original pathogenic strains. A new compound, MAV-1 (1) of the diketopiperazine family (Figure 1) was isolated and identified utilizing these techniques. The structure of MAV-1 (1) was defined by a combination of mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectroscopy. Discovery of MAV-1 (1), a possible precursor to other known compounds, demonstrates the continuing utility of microbial sources with new chemodiversity.
| Identifer | oai:union.ndltd.org:USF/oai:scholarcommons.usf.edu:etd-6988 |
| Date | 16 September 2015 |
| Creators | Veri, Michael |
| Publisher | Scholar Commons |
| Source Sets | University of South Flordia |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Graduate Theses and Dissertations |
| Rights | default |
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