Endogenous phosphorylation was measured in soluble fractions at four stages of Dictyostelium discoideum development. A peak of activity occurred at the slug stage, coincident with the appearance of cyclic AMP-dependent protein kinase. After partial purification by DE-52 cellulose and Sephacryl S-300 chromatography, cyclic AMP dependency of six cytosolic proteins was observed, with apparent subunit molecular weights of > 200,000, 110,000, 107,000, 91,000, 75,000, and 69,000. Phosphorylated bands at 107,000 and 91,000 MW were found to be specific for a partially-purified catalytic subunit prepared from the Dictyostelium holoenzyme form. / M.S.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/105979 |
| Date | January 1983 |
| Creators | Frame, Lynn Teresa |
| Contributors | Zoology |
| Publisher | Virginia Polytechnic Institute and State University |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | viii, 66 leaves, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 10190607 |
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