Hyaluronan is a polymer that among other things is used in fillers. Products containing Hyaluronan is sometimes discoloured over time and the mechanism behind this discolouration is still unknown. However, it was suspected that discolouration occurs during the degradation due to high pH values or with a phosphate buffer. The discolouration of Hyaluronan that occurs with phosphate buffer was studied in more detail in this project. The samples of Hyaluronan with different concentrations of phosphate buffer were left at 90 °C in an oven to speed up the discolouration. These samples were then analyzed byusing UV/Vis spectrophotometry to measure the absorption and capillary viscometry to measure the molecular weight. The results showed that the discolouration increased with time and that the samples with the higher concentration of buffer got more discoloured faster. The molecular weight showed a decreasing trend with time. It also suggested that the phosphate buffer had an impact on the molecular weight. The samples with the highest concentration of phosphate buffer had a lower molecular weight compared to samples with no phosphate buffer. The main conclusion from this study is that the phosphate buffer had an effect on the discolouration of Hyaluronan.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-353782 |
| Date | January 2018 |
| Creators | Fjällström, Alma, Draxler, Emmy, Adan, Saida, Andersson, Sandra, Aidanpää, Louise |
| Publisher | Uppsala universitet, Institutionen för teknikvetenskaper, Uppsala universitet, Institutionen för teknikvetenskaper, Uppsala universitet, Institutionen för teknikvetenskaper, Uppsala universitet, Institutionen för teknikvetenskaper, Uppsala universitet, Institutionen för teknikvetenskaper |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | TVE ; TVE-K 18 003 |
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