Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / O exercÃcio fÃsico de varias intensidades influencia vÃrios sistemas fisiolÃgicos como (neuromuscular) promovendo aumento de forÃa e massa muscular, (cardiovascular)
induzindo bradicardia de repouso e adaptaÃÃes vasculares, endÃcrino favorecendo a liberaÃÃo de vÃrios hormÃnios hipotalÃmicos e atà mesmo o sistema gastrintestinal. O
exercÃcio fÃsico dependendo da intensidade e volume pode levar alteraÃÃes de volemia e isquemia gastrintestinal promovendo alteraÃÃes da motilidade gastrintestinal. O objetivo desse trabalho foi investigar o efeito do exercÃcio fÃsico agudo e crÃnico sobre o esvaziamento gÃstrico de lÃquidos, bem como os possÃveis mecanismos envolvendo o
equilÃbrio Ãcido-bÃsico e as vias neuro-humorais em tais eventos. Utilizamos ratos machos wistas pesando entre 180 a 250g. Os protocolos de exercÃcio foram divididos em
exercÃcio agudo e crÃnico. O agudo consistiu de nataÃÃo em um tanque coletivo 5/dias/10-40min. ApÃs 48h da ultima sessÃo, os ratos foram submetidos a uma sessÃo
aguda de exercÃcio com 5% PC. O exercÃcio crÃnico consistiu de adaptaÃÃo ao meio liquido por 5 dias de nataÃÃo coletiva sem sobrecarga. Quarenta e oito horas apÃs a adaptaÃÃo, dos ratos foram submetidos ao protocolo de exercÃcio de saltos (4x10 intervalo de 30seg, 5dias/semana/4semanas). ApÃs a sessÃo de exercÃcio agudo foram avaliados o EG, gasometria arterial, parÃmetros hemodinÃmicos e mecanismos neurohumorais relacionados aos hormÃnios OT e CCK bem como a expressÃo gÃnica desses hormÃnios em tecidos gastrintestinais. JÃ no exercÃcio crÃnico, foram avaliados o esvaziamento gÃstrico, trÃnsito intestinal, complacÃncia gÃstrica e parÃmetros hemodinÃmicos. Observamos que tanto os exercÃcios fÃsicos agudos quanto crÃnico promoveram diminuiÃÃo significativa (p < 0,05) no esvaziamento gÃstrico de lÃquidos. AlÃm disso, o exercÃcio crÃnico aumentou significativamente (p < 0,05) a complacÃncia gÃstrica em relaÃÃo aos ratos sedentÃrios, sem alteraÃÃo no transito intestinal. Em relaÃÃo aos ratos sedentÃrios, os ratos exercÃcio agudo apresentaram quadro de acidose metabÃlica com diminuiÃÃo significativa (p < 0,05) nos valore de pH, [HCO3]. Tal alteraÃÃo no equilÃbrio Ãcido-bÃsico foi prevenido significativamente (p < 0,05) com o prÃtratamento de NaHCO3 500mg/kg v.o, 40min antes do exercÃcio. Observamos ainda que o prÃ-tratamento com antagonista de OT e de CCK preveniu significativamente (p < 0,05) a diminuiÃÃo do esvaziamento gÃstrico induzido por exercÃcio agudo. O exercÃcio agudo diminuiu significativamente (p < 0,05) os valores relativos na expressÃo gÃnica dos hormÃnios OT e ANP no fundo e piloro dos ratos, quando comparados aos ratos
sedentÃrios. Por outro lado, observamos que o exercÃcio agudo aumentou significativamente (p < 0,05) os valores da expressÃo gÃnica de CCK no fundo, piloro e duodeno dos ratos quando comparados aos sedentÃrios. O exercÃcio fÃsico agudo quanto crÃnico induziu dismotilidade gÃstrica com diminuiÃÃo do esvaziamento gÃstrico. O prÃtratamento
com NaHCO3, Atosibana, Devazepide e Ondansetrona preveniu a diminuiÃÃo do esvaziamento gÃstrico induzido por exercÃcio agudo. Sugerimos que a dismotilidade induzida pelo exercÃcio pode ser influenciada por uma via relacionada a OT, 5-HT e CCK alÃm de sofrer influencia do equilÃbrio acido-bÃsico / In the recent years, there is a general consensus on the benefits of regular physical activity on health caliber, prevention and treatment of various chronic diseases besides improving general quality of life. Specifically, physical activity of varied intensities broadly influence vital physiological systems involving the neuromuscular system (promoting increased muscle strength and mass) cardiovascular (induced basal bradycardia at rest), endocrine (favoring the release of several hypothalamic hormones) and even the gastrointestinal system. Published literature report that depending on the intensity and volume of exercise, changes in blood volume may result to associated gastrointestinal ischemia, which would possibly alter gastrointestinal motility. However, the mechanisms involved in altered gastrointestinal motility due to exercise are yet to be fully elucidated. Thus, the objective of this study was to investigate the effect of acute and chronic exercise on gastric emptying of liquids, as well as explain the possible mechanisms involving acid-base balance and subsequent neurohumoral pathways. Male Wistar rats (180 to 250g), were obtained from the vivarium of the Department of Physiology and Pharmacology, University Federal do Ceara. They were initially assigned to adapt to either acute or chronic exercise protocols. The acute exercise protocol adaptation consisted of collective swimming in a bath tub over increasing scale periods over 5 days, with a single and final swim 48hs after the fifth day, with a 5% body weight load so as to exploit the lactate threshold. The chronic exercise protocol consisted of collective swimming in a bath tub over 5 days without a specific time count pattern. Forty-eight hours after this adaptation, they were submitted to exercise protocol jumps (4x10 30sec interval, 5days/week/4weeks). After a session of intense exercise, we evaluated gastric emptying, blood analysis, hemodynamic and neurohumoral mechanisms related to hormones 5-HT, OT and CCK as well as the gene expression of these hormones in gastrointestinal tissues. In chronic exercised animals, we evaluated gastric emptying, intestinal transit, gastric compliance and hemodynamic parameters. We found that both acute and chronic exercise promoted a significant decrease (p <0.05) gastric emptying of liquids. Moreover, chronically exercised rats had significant (p <0.05) increased gastric compliance, when compared to sedentary rats; but no change in the intestinal transit. When compared to sedentary rats, acute
exercised mice showed metabolic acidosis with a significant decrease (p <0.05) in pH values due to low bicarbonate. This change in acid-base balance was significantly (p <0.05) prevented with pretreating the animals to an oral dose of NaHCO3 (500mg/kg) 40min before exercise. We also observed that pretreatment with OT antagonist and CCK significantly prevented (p <0.05) the decrease in gastric emptying induced by acute exercise. Acute exercise decreased significantly (p <0.05) values for the gene expression of hormones OT and ANP in the fund and pylorus of the rats, when compared to sedentary rats. On the other hand, we found that acute exercise significantly (p <0.05) increased CCK-gene expression values in the fund, pylorus and duodenum of rats, in comparison to sedentary. Concluding, exercise induced changes in gastric motility in both acute and chronic exercise protocols. The pre-treatment with NaHCO3, Atosibana, and Ondansetron Devazepide prevented the decrease in gastric emptying induced by acute exercise. We suggest that dysmotility induced by exercise may be influenced by a pathway related to oxytocin, serotonin and cholecystokinin, which too have a role in the acid-base homeostasis
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:7077 |
| Date | 02 July 2012 |
| Creators | MoisÃs Tolentino Bento da Silva |
| Contributors | ArmÃnio Aguiar dos Santos, Ricardo Brandt de Oliveira, Margaret de Castro, Leandro Masuda Cortonesi, Pedro Jorge Caldas MagalhÃes |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Farmacologia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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