A novel bioassay for the evaluation of plant water status was developed by Burke
(2007). The research reported herein was designed to evaluate this new protocol as a
tool for use in cotton breeding programs for the identification of drought tolerant
genotypes. Twenty genotypes were selected to represent diverse germplasm pools for a
two-year field evaluation. Replicated tests were performed in Lubbock, TX and College
Station, TX in 2005, 2006, and 2007. Dryland and irrigated treatments were
administered in a split plot arrangement of a randomized complete block design.
Fluorescence measurements were taken at mid-bloom and late bloom growth stages of
growth. Source leaf tissue was harvested at predawn and subjected to high temperature
incubation with fluorescence measurements subsequently taken hourly for five hours.
Drought stressed plants had not mobilized their carbohydrate reserves from their source
leaves overnight and thus maintained cell viability and therefore higher chlorophyll
fluorescence values throughout the incubation with the opposite being true for nonstressed
plants. Fiber lint yield and fiber properties were measured at the conclusion of
the 2005 season in College Station and the 2006 season in College Station and Lubbock for comparison with the fluorescence data. Five genotypes, ‘Acala 1517-99’, ‘Deltapine
491’ (PVP no. 200100159), ‘Tamcot CAMD-E’, ‘Tamcot 22’ and TAM 89E-51, an
unreleased breeding line, were selected based on field evaluation results in a preliminary
study in 2005 to be included in a diallel analysis to determine the heritability of
fluorescence measurements. Genotype x treatment effects complicated the classification
of genotypic responses to drought. Few and inconsistent correlations were found among
fluorescence values and lint yield or fiber properties. The diallel analysis did not
identify general combining ability or specific combining ability effects for chlorophyll
fluorescence measurements. Thus this procedure provides little potential in selecting
plants for drought tolerance when plants are grown under field culture. Selection among
Tamcot 22 and TAM 89E-51 plants for high and low genotypes according to
fluorescence values did not yield progeny different from unselected Tamcot 22 and
TAM 89E-51.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2815 |
| Date | 15 May 2009 |
| Creators | Longenberger, Polly Suzanne |
| Contributors | Smith, C. Wayne |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | electronic, application/pdf, born digital |
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