by King Yeung Wong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 143-148). / Abstracts in English and Chinese. / Title Page --- p.i / Acknowledgement --- p.ii / List of Abbreviations --- p.iii / Table of contents --- p.v / Abstract --- p.viii / Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Liver diseases --- p.1 / Chapter 1.2 --- Current treatments of liver diseases --- p.3 / Chapter 1.3 --- Schizandrae --- p.5 / Chapter 1.3.1 --- Chemistry of Schizandrae (Wuweizi) --- p.6 / Chapter 1.3.2 --- Pharmacology of Wuweizi --- p.8 / Chapter 1.3.2.1 --- Hepato-protective effect of Wuweizi --- p.9 / Chapter 1.3.3 --- Toxicology and side-effects of Wuweizi --- p.11 / Chapter 1.4 --- Carbon tetrachloride (CC14) intoxication --- p.12 / Chapter 1.5 --- Hepatic drug metabolism: essential factors --- p.13 / Chapter 1.6 --- Aim --- p.14 / Chapter 2 --- Phase I metabolism --- p.15 / Chapter 2.1 --- Introduction --- p.15 / Chapter 2.2 --- Materials and Methods --- p.18 / Chapter 2.2.1 --- Animals --- p.18 / Chapter 2.2.2 --- Chemicals --- p.18 / Chapter 2.2.3 --- Instruments --- p.19 / Chapter 2.2.4 --- Preparation of Schizandra seed extract --- p.19 / Chapter 2.2.5 --- Animal model of liver damages --- p.20 / Chapter 2.2.6 --- Evaluation of protective effect of Schizandra extract --- p.22 / Chapter 2.2.7 --- Evaluation of healing effect of Schizandra extract --- p.24 / Chapter 2.2.8 --- Extraction of antipyrine from blood and urine --- p.26 / Chapter 2.2.9 --- TLC method for quantitative analysis of antipyrine --- p.26 / Chapter 2.2.10 --- Analysis of pharmacokinetic parameters of antipyrine --- p.27 / Chapter 2.2.11 --- Statistical analysis --- p.28 / Chapter 2.3 --- Results --- p.30 / Chapter 2.3.1 --- Effect of CCI4 and Schizandra seed extract on antipyrine metabolism --- p.30 / Chapter 2.4 --- Discussion --- p.41 / Chapter 3 --- Phase II metabolism --- p.44 / Chapter 3.1 --- Introduction --- p.44 / Chapter 3.2 --- Materials and Methods --- p.46 / Chapter 3.2.1 --- Chemicals --- p.46 / Chapter 3.2.2 --- Preparation of Schizandra extract --- p.46 / Chapter 3.2.3 --- Preparation of Salicylamide solution (for injection) --- p.47 / Chapter 3.2.4 --- Preparation of 2，4-dinitrophenylhydrazine solution --- p.47 / Chapter 3.2.5 --- Animal groups --- p.47 / Chapter 3.2.6 --- Animal model of liver damage --- p.48 / Chapter 3.2.7 --- Evaluation of the hepato-protective effect of Schizandra extract --- p.49 / Chapter 3.2.8 --- Determination of serum glutamate pyruvate transaminase (SGPT/ALT) and serum glutamate oxaloacetate transaminase (SGOT/AST) --- p.50 / Chapter 3.2.9 --- Salicylamide adminstration and plasma collection --- p.51 / Chapter 3.2.10 --- Procession of plasma and urine samples --- p.52 / Chapter 3.2.11 --- HPLC Analysis --- p.54 / Chapter 3.2.12 --- Preparation of liver microsomes --- p.55 / Chapter 3.2.13 --- Determination of cytochrome P450 --- p.56 / Chapter 3.2.14 --- Determination of protein content of the liver microsomes --- p.57 / Chapter 3.2.15 --- Data Analysis --- p.58 / Chapter 3.2.16 --- Statistical Analysis --- p.58 / Chapter 3.3 --- Results --- p.60 / Chapter 3.3.1 --- Liver enzyme levels --- p.60 / Chapter 3.3.2 --- Phase II metabolism profile of salicylamide --- p.61 / Chapter 3.3.3 --- Cytochrome P450 content of liver --- p.64 / Chapter 3.4 --- Discussion --- p.65 / Chapter 3.4.1 --- Liver enzyme assay --- p.65 / Chapter 3.4.2 --- Cytochrome P450 activity --- p.67 / Chapter 3.4.3 --- Hepatic metabolism of salicylamide --- p.68 / Chapter 3.4.4 --- Effect of CC14 intoxication on Phase II metabolism --- p.71 / Chapter 3.4.5 --- Wuweizi actions on Phase II metabolism --- p.73 / Chapter 4 --- Protein binding --- p.102 / Chapter 4.1 --- Introduction --- p.102 / Chapter 4.2 --- Materials and Methods --- p.104 / Chapter 4.2.1 --- Chemicals --- p.104 / Chapter 4.2.2 --- Instruments --- p.105 / Chapter 4.2.3 --- Preparation of Warfarin sodium solution --- p.105 / Chapter 4.2.4 --- Animal groups --- p.106 / Chapter 4.2.5 --- Equilibrium dialysis --- p.106 / Chapter 4.2.5.1 --- Equilibration time --- p.106 / Chapter 4.2.5.2 --- Equilibrium dialysis of different warfarin concentration --- p.107 / Chapter 4.2.6 --- High performance liquid chromatography analysis of warfarin --- p.108 / Chapter 4.2.7 --- Calibration curve --- p.109 / Chapter 4.3 --- Results --- p.111 / Chapter 4.3.1 --- Equilibriation time --- p.111 / Chapter 4.3.2 --- Calibration curve --- p.111 / Chapter 4.3.3 --- Free concentration of warfarin --- p.112 / Chapter 4.4 --- Discussion --- p.114 / Chapter 4.4.1 --- Effect of CCl4 intoxication on free percentage of warfarin --- p.114 / Chapter 4.4.2 --- Effcct of wuweizi cxtract on free percentage of warfarin --- p.115 / Chapter 4.4.2.1 --- Depletion of plasma albumin concentration --- p.116 / Chapter 4.4.2.2 --- Displacement of warfarin by WWZ extract --- p.117 / Chapter 4.4.3 --- Concentration dependent protein binding --- p.118 / Chapter 5 --- Hepatic blood flow --- p.124 / Chapter 5.1 --- Introduction --- p.124 / Chapter 5.2 --- Materials and Methods --- p.126 / Chapter 5.2.1 --- Chemicals....: --- p.126 / Chapter 5.2.2 --- Instruments --- p.126 / Chapter 5.2.3 --- Preparation of indocyanine green (ICG) solution --- p.126 / Chapter 5.2.4 --- Preparation of Schizandra seed extract --- p.127 / Chapter 5.2.5 --- Animals groups --- p.127 / Chapter 5.2.6 --- Animal model of liver damage --- p.128 / Chapter 5.2.7 --- Evaluation of hepato-protective effect of Schizandra extract --- p.129 / Chapter 5.2.8 --- Evaluation of healing effect of Schizandra extract --- p.129 / Chapter 5.2.9 --- Quantitative analysis of ICG in plasma by UV spectroscopy --- p.130 / Chapter 5.2.10 --- Analysis of pharmacokinetic parameters of ICG --- p.131 / Chapter 5.2.11 --- Statistical analysis --- p.132 / Chapter 5.3 --- Results --- p.133 / Chapter 5.4 --- Discussion --- p.135 / Chapter 5.4.1 --- Effect of CCl4 intoxication on hepatic blood flow --- p.135 / Chapter 5.4.2 --- Effect of WWZ pretreatment on hepatic blood flow --- p.135 / Chapter 5.4.3 --- Effect of WWZ healing on hepatic blood flow --- p.136 / Chapter 6 --- General conclusion --- p.139 / Significance of the study --- p.141 / References --- p.143
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322876 |
| Date | January 1999 |
| Contributors | Wong, King Yeung., Chinese University of Hong Kong Graduate School. Division of Pharmacy. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 148 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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