Human tumor susceptibility gene 101, TSG101, exhibits a variety of functions including protein sorting, vesicular trafficking, and regulation of transcription, epithelial growth and differentiation. The upstream sequence of TSG101 gene shows a typical housekeeping TATA-less and Sp1 containing promoter. Our previous data indicated the essential role of TSG101 in skin keratinocyte differentiation that is under the regulation of PKC-Sp1 signaling. In this report, we investigated the correlation of TSG101 and Sp1 expression in the specimens of cervical intraepithelial neoplasia. Cervical intraepithelial neoplasia specimens used in this study were 129 paraffin blocks from 41 normal, 35 CIN I, 28 CIN II and 25 CIN III/CIS patients collected in Cancer Prevention and Screening Center at Kaohsiung from January 2005 to July 2007. The expression of TSG101 and Sp1 were analyzed by immunohistochemistry and digitally quantified by Image Pro-plus 6.1 Microimage software according to the method described by Eliane Pedra Dias et al. The quantified data were statistically analyzed using Spearman's rho coefficient and SPSS for Win, v.14 statistical software (SPSS Inc., Chicago, USA). Values were considered significantly different when the P value < 0.05. We found that TSG101 and Sp1 are expressed in cells of parabasal and intermediate layers in normal cervical epithelium, whereas their expressions in basal and superficial layers were either absence or reduced. Interestingly, the expressions of these two markers are significantly increased in more advanced progression stages (CIN II and CIN III/CIS) of cervical intraepithelial neoplastic specimens (P < 0.05). Congruous expression pattern of TSG 101 and Sp1 in normal cervical epithelium confirms the important of cellular Sp1 signaling in regulating TSG101 expression, which is essential during epithelial cell growth and differentiation. Our results also indicate upregulation of these two markers might be important for the progression of cervical intraepithelial neoplasia. Further analysis using more specimens should reveal the prognostic value of these two markers.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0825108-115834 |
| Date | 25 August 2008 |
| Creators | Huang, Chia-wen |
| Contributors | Jiin-tsuey Cheng, Chee-yin Chai, Yun-nan Lin |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825108-115834 |
| Rights | withheld, Copyright information available at source archive |
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