NMDA (N-methyl D-aspartate) Receptor is a ligand and voltage gated ion channel and
involved in many processes such as synaptic plasticity, memory formation, behavioral
responses and cell survival. In the sense of functional activity, cellular localization of NMDAR
is important since this receptor shows its activity on the membrane. Although NMDA receptor
is intensely studied there are no satisfying study showing its localization with microscobic
methods. Besides, the effect of florescent protein labelling of NMDA receptor on its
distribution is not shown. It is expected to provide basis for further interaction and distribution
studies with this comparison.
Contrary to literature, in this study it is shown that NMDA receptor does not localize only in
ER and membrane instead has a cytosolic pattern and this pattern is compatible with the
distribution of wild type NMDA receptor. In addition, florescent protein labelling of NMDA
receptor does not interrupt cellular distribution of NMDAR. Moreover, this study shows that
N-terminal domain of NR1 subunit is sufficient to prevent degradation of NR2B in the cell.
In consideration of this study it can be concluded that EGFP and mCherry labelled NMDA
receptors can be used in interaction studies such as FRET and other studies, making use of
fluorescent labelling of NMDA receptors, in terms of cellular distribution.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12615384/index.pdf |
| Date | 01 January 2013 |
| Creators | Pirincci, Serife Seyda |
| Contributors | Son, Cagdas Devrim |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for METU campus |
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