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Brassinosteroids confer tolerance to plants under the nitrogen (N) starvation stress by enhancing low-N induced anthocyanin biosynthesis.

Jiang, Tiantian. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 61-75). / Abstracts in English and Chinese. / Thesis/Assessment Committee --- p.ii / Statement --- p.iii / Abstract --- p.iv / 摘要 --- p.v / Acknowledgements --- p.vi / List of Figures and Tables --- p.vii / Chapter Part 1 --- Introduction --- p.-0- / Chapter 1.1 --- Brassinosteriods (BRs) and BR signaling --- p.-0- / Chapter 1.1.1 --- Discovery of BRs --- p.-2- / Chapter 1.1.2 --- Functions of BRs --- p.-4- / Chapter 1.1.3 --- BR signaling pathway --- p.-6- / Chapter 1.2 --- Nitrogen (N) and N responses --- p.-10- / Chapter 1.2.1 --- Hormones involved in plant N responses --- p.-11- / Chapter 1.3 --- Anthocyanin and anthocyanin synthesis --- p.-13- / Chapter 1.3.1 --- Anthocyanin structures --- p.-13- / Chapter 1.3.2 --- Functions of anthocyanins --- p.-14- / Chapter 1.3.3 --- Biosynthesis of anthocyanins --- p.-14- / Chapter 1.3.4 --- Regulations of anthocyanin biosynthesis --- p.-15- / Chapter 1.4 --- Hormones and plant nutrient stresses --- p.-19- / Chapter Part 2 --- Materials and Methods --- p.-20- / Chapter 2.1 --- Plant materials and growth conditions --- p.-20- / Chapter 2.2 --- Measurement of anthocyanin content --- p.-21- / Chapter 2.3 --- Yeast two-hybrid (Y2H) assay --- p.-22- / Chapter 2.4 --- Bimolecular fluorescence complementation (BiFC) assays --- p.-23- / Chapter 2.5 --- Quantitative real-time PCR --- p.-25- / Chapter 2.6 --- Electrophoretic mobility shift assay (EMSA) and competition assay --- p.-26- / Chapter 2.7 --- Histochemical staining of GUS activity --- p.-28- / Chapter Part 3 --- Results --- p.-29- / Chapter 3.1 --- 24-epibrassinolide (24-eBR) increases plant tolerance to N-starvation in Arabidopsis - --- p.-29- / Chapter 3.2 --- BR treatment enhances anthocyanin accumulation under N deprivation conditions --- p.-31- / Chapter 3.3 --- BZR1 interacts with PAP1 in vitro and in vivo --- p.-35- / Chapter 3.4 --- BR and BZR1 promote the expression of the 'late' anthocyanin biosynthetic genes during N deprivation - --- p.-39- / Chapter 3.5 --- BZR1 binds to the promoter of DFR --- p.-43- / Chapter 3.6 --- BR-enhanced anthocyanin accumulation is specific to N-deprivation --- p.-46- / Chapter 3.7 --- BZR1 differently regulates PAP1 and PAP2 --- p.-48- / Chapter 3.8 --- Endogenous GL3 is required for BR-enhanced anthocyanin biosynthesis --- p.-52- / Chapter 3.9 --- N status affects the expression of BR biosynthetic gene CPD --- p.-52- / Chapter Part 4 --- Discussion --- p.-54- / Chapter 4.1 --- BRs confer plant tolerance to low-N stress and the tolerance is mediated by BR enhancement of low-N-induced anthocyanin biosynthesis --- p.-54- / Chapter 4.2 --- BRs enhance anthocyanin accumulation under N starvation through BZR1-PAP1 interaction or direct control of the expression of anthocyanin biosynthetic genes --- p.-55- / Chapter 4.3 --- BRs are specifically involved in low-N induced anthocyanin production --- p.-56- / Chapter 4.4 --- Transcription factors that specifically control BR-regulated anthocyanin biosynthesis --- p.-57- / Chapter 4.5 --- DFR is an important target of BR-regulation of anthocyanin biosynthesis --- p.-58- / Chapter Part 5: --- Conculsions --- p.-59- / Chapter Part 6: --- References --- p.-61-

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327364
Date January 2011
ContributorsJiang, Tiantian., Chinese University of Hong Kong Graduate School. Division of Life Sciences.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, ix, 75 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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