This diploma thesis deals with the influence of tree dieback as a result of bark beetle attack on the ectomycorrhizal fungi community associated with the spruce seedlings. Seedlings were collected from forests located in three different permanent plots in the Tatra National Park, with different intensity of bark beetle attack (K1, K2, REF). Their biometric characteristics were measured and their micro-site conditions was described. The differences between the measured biometric parameters of the seedlings from the individual areas and the number of ectomycorrhizal species per seedling were tested by the scattering analysis in the Statistica program. Ectomycorrhizas from seedling roots have been categorized by morphological attributes into morphotypes; fungi generating these morphotypes were identified by comparison of the ITS rDNA sequences. Using the Canoco statistical program, it was found, which factors (micro-sites, biometric parameters) had influence on the ectomycorrhizal fungal community on seedlings, and whether it is related to the intensity of the bark beetle attack on forest. Seedlings from dead forest (K2) reached significantly higher elevations of aboveground part, root collar thickness, and had longer main root than the seedlings collected from healthy forest (REF), where on the contrary were the seedlings older (higher number of nodes). The type of area (intensity of bark beetle attack) and age were demonstrated as proof parameters in the Canoco program by CCA analysis in relation to the occurrence of ectomycorrhizal species on seedlings. By analyzing of RDA in relation to the occurrence of ectomycorrhizal species on seedlings classified into exploration types, the type of the area (intensity of bark beetle attack) and the micro-location of seedlings has shown as proving. The ectomycorrhizal fungal community associated with the spruce seedlings has been influenced to a certain extent by the state of the forest, however, the interpretation of these differences is difficult because there is a lack of data on ecology and physiology of individual ectomycorrhizal species.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:428070 |
| Date | January 2018 |
| Creators | Loubalová, Karolína |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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