Bisphenol A (BPA), an endocrine disruptor, requires monitoring in water for health safety. Glutamate, H2O2, and glucose are vital biomarkers for various diseases. However, lab-based methods are expensive, time-consuming, and require skilled personnel, making them unsuitable for point-of-care (POC) devices. The electrochemical sensor enables POC device development. However, it suffers from low sensitivity and selectivity. This thesis focuses on the use of nanomaterials to enhance the sensitivity and selectivity of electrochemical sensors to monitor BPA in water, along with glutamate, H2O2, and glucose in bio-fluids.
A BPA sensor was developed using chemically modified MWCNTs with βCD on a screen-printed carbon electrode (SPCE). The MWCNTs-βCD/SPCE exhibited high sensitivity, attributed to the catalytic activity of MWCNTs and the host-guest interaction ability of βCD. It provided a linear range (LR) of 125 nM −30 µM, with a limit of detection (LOD) of 13.76 nM (SNR = 3). We improved the performance by curing the MWCNTs-βCD/SPCE with CTAB. The sensor demonstrated a dynamic range of 500 fM to 10 μM, with a LOD of 96.5 fM, surpassing the Canada-assigned PNEC of BPA in water (0.77 nM).
We fabricated a nonenzymatic glutamate sensor using CuO nanostructures and MWCNTs on SPCE. The sensor showed irreversible oxidation of glutamate involving one electron and one proton, and an LR of 20 μM−200 μM with LOD of 17.5 μM and sensitivity of 8500 μAmM−1cm−2. The sensor is promising to detect glutamate in blood.
We developed a nonenzymatic glucose sensor using green synthesized gold nanoparticles and CuO-modified SPCE. The LR offered by the sensor (2 µM to 397 µM) is suitable for quantifying saliva glucose. We also created nonenzymatic H2O2 sensor by green synthesized silver nanoparticles modified SPCE which offers LR of 0.5- 161.8 µM with LOD 0.3 µM which is capable of H2O2 monitoring in urine. / Thesis / Doctor of Philosophy (PhD) / Bisphenol A (BPA) is a plastic pollutant and an endocrine-disrupting chemical that causes reproductive and neurodevelopmental disorders, and many diseases including obesity, diabetes, and cardiovascular disease. In addition, glutamate, hydrogen peroxide (H2O2), and glucose are vital biomarkers for various acute and chronic diseases. These diseases impose significant burdens on individuals, healthcare systems, and the economy. Therefore, they must be monitored. In this thesis, we developed a BPA sensor using chemically modified multiwall carbon nanotubes (MWCNTs) with β-cyclodextrin (βCD) and cetrimonium bromide (CTAB) which can detect BPA at very low concentration beyond Canada-assigned predicted-no-effect-concentrations (PNEC) of BPA. We also developed a glutamate sensor using MWCNTs and wet chemically synthesized copper oxide (CuxO) nanostructure which offers a linear range related to blood glutamate level. Moreover, we fabricated nonenzymatic H2O2 and glucose sensors using green synthesized gold (AuNPs) and silver (AgNPs) nanoparticles (using orange peel extraction as a reducing and stabilizing agent) which are useful to quantify urine H2O2 and saliva glucose respectively.
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/28874 |
| Date | January 2023 |
| Creators | Ali, Md Younus |
| Contributors | Howlader, Matiar M. R., Electrical and Computer Engineering |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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