Photo-polymerized cross-linked polyacrylamide gels are becoming increasingly
important for use in micro-fabricated DNA electrophoresis systems because they allow a
concentrated sieving matrix to be precisely positioned at any location within a complex
micro-channel network. The rate of photo-initiation in the free radical gel polymerization
reaction, however, can exert a strong influence on the resulting gel structure.
Experimental data on separation resolution of single stranded DNA (ssDNA) in photoinitiated
polyacrylamide gels is very sparse. In this study, we investigate the
performance of ssDNA electrophoresis in an ALF Express automated DNA sequencer
using various photo-initiation chemistries. Cross-linked polyacrylamide gels with
concentrations ranging from 6 to 12 %T were prepared using riboflavin, methylene blue,
irgacure 651 and ReproGel (AP Biotech) photo-initiators. Separation resolution is
compared with that attained in cross-linked polyacrylamide gels prepared using
conventional chemical initiators (e.g. ammonium persulfate/
Tetramethylethylenediamine) in order to determine the polymerization conditions
necessary for optimum performance.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/2311 |
| Date | 29 August 2005 |
| Creators | Agrawal, Shilpa |
| Contributors | Ugaz, Victor |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 4333283 bytes, electronic, application/pdf, born digital |
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