The electrophysiological and morphological properties of small networks of mammalian neurons were investigated with mouse spinal cord monolayer cultures of 2 mm diameter grown on multimicroelectrode plates (MMEPs). Such cultures were viewed microscopically and their activity simultaneously recorded from 2 of any 36 fixed recording sites. The specific aims achieved were: development of techniques for production of functional MMEPs and maintenance of mini-cultures, characterization of the spontaneous activity of mini-cultures, application of inhibitory and disinhibitory agents, development of staining methods for cultured neurons and initial light microscopic analysis with correlation of electrophysiological and morphological characteristics.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798142 |
| Date | 12 1900 |
| Creators | Hightower, Mary H. (Mary Helen) |
| Contributors | Gross, Guenter W., Wu, Edward Ming-chi, 1938-, Donahue, Manus J., Droge, Michael H., Cook, Paul F. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | xi, 311 leaves : ill., Text |
| Rights | Public, Hightower, Mary H. (Mary Helen), Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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