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Electrophysiological and Morphological Analyses of Mouse Spinal Cord Mini-Cultures Grown on Multimicroelectrode Plates

The electrophysiological and morphological properties of small networks of mammalian neurons were investigated with mouse spinal cord monolayer cultures of 2 mm diameter grown on multimicroelectrode plates (MMEPs). Such cultures were viewed microscopically and their activity simultaneously recorded from 2 of any 36 fixed recording sites. The specific aims achieved were: development of techniques for production of functional MMEPs and maintenance of mini-cultures, characterization of the spontaneous activity of mini-cultures, application of inhibitory and disinhibitory agents, development of staining methods for cultured neurons and initial light microscopic analysis with correlation of electrophysiological and morphological characteristics.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798142
Date12 1900
CreatorsHightower, Mary H. (Mary Helen)
ContributorsGross, Guenter W., Wu, Edward Ming-chi, 1938-, Donahue, Manus J., Droge, Michael H., Cook, Paul F.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatxi, 311 leaves : ill., Text
RightsPublic, Hightower, Mary H. (Mary Helen), Copyright, Copyright is held by the author, unless otherwise noted. All rights

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