In this study, the production of monoclonal antibodies directed against the activating enzyme for an S6 kinase is examined and described. Evidence is presented for the association of an Mr. 55,000 abd Mr. 95,000 protein with the s6 kinase. These proteins are phosphorylated in the presence of Activating Enzyme. A sequence of regulatory events for insulin-stimulated phosphorylation of ribosomal protein S6 in cells is postulated as follows: insulin activates the receptor tyrosine kinase, which phosphorylates the Mr 116,000 subunit of Activating Enzyme. The Activating Enzyme then activates the S6 kniase by phosphorylation, and phosphorylation of the ribosomal protein s6 is promoted.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798366 |
| Date | 05 1900 |
| Creators | Murdoch, Fern E. (Fern Elizabeth) |
| Contributors | Masaracchia, Ruthann A., Jacobson, Myron, Pirtle, Robert M., Wu, Edward, Harris, Ben G. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | ix, 186 leaves : ill., Text |
| Rights | Public, Murdoch, Fern E. (Fern Elizabeth), Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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