Abstract
The contribution of the volatile constituents to the overall antimicrobial efficacy of the medicinal plant Tarchonanthus camphoratus was
considered, where different extraction techniques were applied to yield four fractions. These comprised of the essential oil prepared by
hydrodistillation, non-volatile constituents prepared by extraction of plant material remaining in the distilling apparatus (having no or negligible
volatile constituents), and extracts prepared from fresh and dried plant material having both volatile and non-volatile constituents. The
antimicrobial activities of the non-volatile and volatile fractions of T. camphoratus singularly (MIC method) and in combination (isobologram
ratio method) demonstrated that the volatile constituents play an integral role in the total antimicrobial efficacy of the plant. The MIC values for
the essential oils of T. camphoratus ranged from 1.5 to 16.0 mg/ml depending on the pathogen studied. With the exception of studies on
Klebsiella pneumoniae, the non-volatile fraction devoid of volatile constituents displayed higher antimicrobial efficacies (2.0–4.0 mg/ml). When
the volatile and non-volatile fractions were combined, increased efficacy was mostly noted with the dried plant material mostly showing a higher
antimicrobially-active profile. Synergistic interactions were further validated by the isobologram studies on the combination of non-volatiles with
essential oil.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1001729 |
| Date | 28 July 2009 |
| Creators | Van Vuuren, SF, Viljoen, A M |
| Publisher | Elsevier |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | |
| Rights | © 2009 SAAB |
| Relation | South African Journal of Botany |
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