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Previous issue date: 2017-09-29 / O Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A S?rie Percomorpha ? maior divis?o entre os vertebrados, constituindo o maior e mais
derivado clado de peixes tele?steos. Dentre suas Ordens, Perciformes e Labriformes
constituem modelos adequados ? investiga??o do conservadorismo e diversifica??o
cromoss?mica. Em Perciformes o conservadorismo cromoss?mico ? representado por
cari?tipos basais com 2n=48 acroc?ntricos, extensivamente compartilhados por uma parcela
consider?vel de esp?cies. As causas e extens?o do conservadorismo cariot?pico em v?rias
fam?lias desta Ordem n?o s?o inteiramente claras. Diante da diversidade de esp?cies em
Labriformes, aspectos mais detalhados de sua evolu??o cariot?pica ou mesmo status
taxon?mico de algumas esp?cies merecem particular aten??o. Com vistas a contribuir com
novas informa??es sobre essas quest?es foram implementadas an?lises cromoss?micas
convencionais (colora??o convencional com Giemsa, bandamento C e Ag-RONs,
fluocrocromos base-espec?ficos) e citomoleculares (hibridiza??o in situ com sondas DNAr
18S, DNAr 5S). Oito esp?cies dos g?neros Anisotremus e Haemulon da fam?lia Haemulidae
(Perciformes) foram analisadas, incluindo amostras de diferentes ?reas do Atl?ntico, como
modelo de evolu??o conservativa. Em 2 esp?cies do g?nero Bodianus da fam?lia Labridae
(Labriformes), foram analisados tamb?m aspectos da evolu??o de sequ?ncias repetitivas
particulares (DNAr 18S, DNAr 5S, Alu e Tol2) nos cromossomos por hibridiza??o com 5
metilcitosina (5mC). Adicionalmente, foram realizadas compara??es filogen?ticas, utilizando
sequ?ncias de DNA mitocondrial (COI e 16S) e nuclear (Rodopsina) para verificar o status
taxon?mico da esp?cie Bodianus insularis, end?mica das ilhas Meso-Atl?nticas. Todas as
esp?cies de Haemulidae apresentaram 2n=48a, s?tios Ag-RONs simples e heterocromatina
centrom?rica reduzida, al?m de consider?vel compartilhamento de s?tios de DNAr 5S e 18S,
confirmando a ocorr?ncia de estase cariot?pica na fam?lia. Os padr?es cariot?picos das
popula??es de A. virginicus e H. chrysargyreum entre o Caribe e Atl?ntico Sul n?o revelaram
varia??es cromoss?micas decorrentes da barreira dos des?gues dos rios Amazonas/Orinoco.
Em Bodianus, as an?lises identificaram uma not?vel regi?o descondensada em um par
cromoss?mico subteloc?ntrico, denominada regi?o BOD. Entre suas caracter?sticas
constitutivas e funcionais particulares, se mostram DAPI-, argentof?lica (Ag+),
marcantemente hipometilada e saturada de elementos transpon?veis, sugerindo que a
participa??o destes elementos m?veis pode ter contribu?do para sua g?nese e din?mica
epigen?tica complexa. Quanto a esp?cie end?mica B. insularis, sua diverg?ncia gen?tica ?
muito inferior ? apresentada por esp?cies diferenciadas sugerindo que embora represente
um grupo geograficamente isolado, constitua uma sinon?mia de B. pulchellus. A diverg?ncia
nos ritmos de diversifica??o cariot?pica entre Haemulidae e Labridae ? aqui discutida ? luz de
caracter?sticas cariot?picas intr?nsecas que podem favorecer o tamponamento e a fixa??o de
mudan?as cromoss?micas e aspectos biol?gicos das esp?cies que contribuem para as
condi??es particulares de evolu??o cariot?pica desses dois grupos de peixes marinhos. / Percomorpha series is the largest division among vertebrates, constituting the largest and
most derived clade of teleostean fishes. Among its Orders, Perciformes and Labriformes
constitue adequated models for the investigation of conservatism and chromosomal
diversification. In Perciformes the chromosomal conservatism is represented by basal
karyotypes with 2n = 48 acrocentric, extensively shared by a considerable portion of species.
The causes and extent of the karyotypic conservatism in various families of this Order are not
entirely clear. In front of the diversity of species in Labriformes, more detailed aspects of its
karyotype evolution or even the taxonomic status of some species deserve particular
attention. In order to contribute with new information on these issues, it was implemented
conventional chromosome analysis (conventional staining with Giemsa, C-banding and Ag-
NORs, base-specific fluochromes) and cytomolecular (in situ hybridization with 18S rDNA, 5S
rDNA) probes were implemented. Eight species of the genera Anisotremus and Haemulon of
the family Haemulidae (Perciformes) were analyzed, including samples from different areas of
the Atlantic, as a model of conservative evolution. In 2 species of the Bodianus genus
Labridae (Labriformes), it was also analyzed aspects of the evolution of particular repetitive
sequences (DNAr 18S, DNAr 5S, Alu and Tol2) in the chromosomes by hybridization with 5
methylcytosine (5mC). Additionally, phylogenetic comparisons were performed using
mitochondrial (COI and 16S) and nuclear (Rhodopsin) DNA sequences to verify the
taxonomic status of the Bodianus insularis species, endemic to the Meso-Atlantic islands. All
Haemulidae species presented 2n = 48a, single Ag-NORs sites and reduced centromeric
heterochromatin, besides considerable sharing of rDNA 5S and 18S sites, confirming the
occurrence of karyotype stasis in the family. The karyotypic patterns of the populations of A.
virginicus and H. chrysargyreum between the Caribbean and the South Atlantic did not reveal
chromosomal variations due to the barrier of the discharges of the Amazonas / Orinoco rivers.
In Bodianus, the analyzes identified a remarkable decondensed region in a subtelocentric
chromosomal pair, denominated BOD region. Among its particular constitutive and functional
characteristics, it is DAPI-, Argentof?lica (Ag +), markedly hypomethylated and saturated with
transposable elements, suggesting that the participation of these mobile elements may have
contributed to its genesis and complex epigenetic dynamics. In relation to the endemic
species B. insularis its genetic divergence is much inferior to that presented by differentiated
species suggesting that although it represents a geographically isolated group, it constitutes a
synonym of B. pulchellus. The divergence in the rates of karyotype diversification between
Haemulidae and Labridae is discussed here in the light of intrinsic karyotype characteristics
that may favor the buffering and the fixation of chromosomal changes and biological aspects
of the species that contributes to particular conditions of karyotype evolution of these two
groups of marine fish.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24954 |
| Date | 29 September 2017 |
| Creators | Motta Neto, Cl?vis Coutinho da |
| Contributors | 56615043491, Souza, Allyson Santos de, 00947093435, Garcia J?nior, Jos?, 27234944893, Cioffi, Marcelo de Bello, 07333907651, Lima Filho, Paulo Augusto de, 30720553865, Artoni, Roberto Ferreira, Molina, Wagner Franco |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM SISTEM?TICA E EVOLU??O, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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