Endocannabinoid and immune system alterations at moderate (18 m/min)
and endurance (32 m/min) exercise intensities were assessed and compared to
controls. Rats were exercised for 60 minutes/day, 5 days/week for 16 weeks.
Immune effector cell proportions (T cell subtypes, B cells, NK cells, and
neutrophils) and endocannabinoid serum levels were determined. Anandamide
(ANA) and 2 arachidonyl-glycerol (2-AG) serum levels increased with
endurance type of exercise. mRNA expression of the CB1 receptor increased
together with ANA in the same group. Apoptotic index increased while immune
effector cells responded divergently. B lymphocyte percentage decreased while
T lymphocyte and NK cell percentage increased in blood. CD8+ subtypes
increased whereas CD11b+ cell and CD25+ cell numbers decreased in the spleen
in the endurance type of exercise group.
Rats were grouped as the control, the endurance type of exercise, the
AM281 (CB1 receptor antagonist) and the AM281+AM630 (CB2 receptor
antagonist) groups in the second part of the study. Flow cytometry and
microarray analyses of the spleen and the thymus were conducted. Endurance
type of exercise associated significantly to immunological changes particularly
to that of the T lymphocytes. T lymphocytes increased whereas cytolytic T
lymphocytes decreased in blood. T cell and double positive T cell percentages
significantly increased in the spleen. Activated T cells and NK like T cells furthermore decreased in the spleen. AM281 and/or AM630 could partially
reverse the effect of exercise in blood but not in the spleen. Alterations in the
thymus were not observed. Exercise altered 302 genes, some of them related
with the immune system. Up-regulation of heat-shock protein coding genes was
the most significant ones.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12611362/index.pdf |
| Date | 01 November 2009 |
| Creators | Ozdurak, Rabia Hurrem |
| Contributors | Korkusuz, Feza |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | Ph.D. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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