Skeletal muscle damage can lead to cell death and the subsequent release of intracellular molecules. To investigate whether molecules from skeletal muscle can interact with the heart, the Langendorff isolated heart preparation was used to assess cardiac function, while NF-κB and AP-1 activation were assessed by EMSA to observe inflammatory status. Hearts were perfused for 75 min with 1 μg/ml of either soleus, white gastrocnemius (WG), or heat stressed white gastrocnemius (HSWG) skeletal muscle homogenate and a decreased LVDP, +dP/dt and –dP/dt were observed when compared to untreated (control) hearts. Greater early and late decreases in cardiac function were observed in hearts treated with HSWG and soleus muscle homogenates, respectively. No alterations in NF-κB or AP-1 activation were detected. These data suggests the contents of skeletal muscle are capable of interacting with the heart and altering contractile function in a fibre type specific manner, possibly related to muscle HSP content.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/27331 |
| Date | 30 May 2011 |
| Creators | Di Battista, Alex |
| Contributors | Locke, Marius |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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