In vitro cell culture is an essential part of many cell and tissue engineering approaches. In particular, monolayer culture of mammalian cells is a key tool for applications such as cell therapy. Novel bioreactors like the Cellerator(TM) allow for expansion of cell populations on mechanically stimulated surfaces coated with proteins. This thesis constitutes a preliminary study which focused on cell-matrix interactions in the absence of stretch. The aim was to establish standard protocols for protein coating on poly (dimethyl siloxane) (PDMS) and for measuring cell proliferation. Specifically, the proliferation of rat pulmonary artery vascular smooth muscle (PAC1) cells on type I collagen and soluble fibronectin was studied. Growth curves were obtained and the doubling time for subconfluent cultures was computed. Although cell-matrix interactions do not enhance proliferation of PAC1 cells, it was found that a preliminary sulphuric acid treatment is necessary to yield a well-behaved culture.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.116017 |
| Date | January 2008 |
| Creators | Lavoie, Jean-Michel. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Engineering (Department of Chemical Engineering.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002840041, proquestno: AAIMR66953, Theses scanned by UMI/ProQuest. |
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