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Previous issue date: 2015-06-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A 3-Hidroxitiramina/Dopamina (DA) ? uma monoamina do grupo dascatecolaminas e consiste na subst?ncia precursora da s?ntese de noradrenalina eadrenalina, tendo a enzima Tirosina-Hidroxilase (TH) como reguladora desse processo.Al?m disso, a DA tem a capacidade de atuar como neurotransmissor no SistemaNervoso Central - SNC, sendo o neurotransmissor principal de neur?nios de noven?cleos encef?licos, nomeados de A8 ao A16. Os n?cleos do mesenc?falo queexpressam DA s?o a Zona Retrorubral (RRF, grupo A8), a Subst?ncia Negra parscompacta (SNc, grupo A9) e a ?rea Tegmental Ventral (VTA, grupo A10). Tais n?cleosest?o envolvidos em tr?s complexas circuitarias que s?o a mesoestriatal, mesol?mbica emesocortical, os quais est?o relacionadas diretamente com diversas manifesta??escomportamentais como controle da motricidade, sinaliza??o de recompensa naaprendizagem comportamental, motiva??o e nas manifesta??es patol?gicas da Doen?ade Parkinson e esquizofrenia. Todavia, muitos aspectos de car?ter morfofuncionaldesses n?cleos ainda continuam sem esclarecimentos. Considerando a relev?ncia dosn?cleos dopamin?rgicos mesencef?licos, o objetivo deste trabalho ? caracterizarmorfologicamente os n?cleos dopamin?rgicos (grupamentos A8, A9 e A10) domesenc?falo do morcego (Artibeus planirostris). O Artibeus planirostris ? um morcegocomum no Rio Grande do Norte. Foram utilizados dez animais nesta pesquisa. Osanimais foram anestesiados, perfundidos e os enc?falos removidos da cavidadecraniana. Ap?s desidrata??o em sacarose, os enc?falos foram submetidos a microtomiae sec??es coronais foram obtidas e coletadas em seis compartimentos distintos. Oscompartimentos foram submetidos a colora??o pela t?cnica de Nissl para an?lisecitoarquitet?nica e as demais s?ries foram submetidas a imunoistoqu?mica para TH.Com base na t?cnica de Nissl e na imunoistoqu?mica para TH foi poss?vel verificar oslimites anat?micos, assim como a citoarquitetura e poss?veis subdivis?es dos tr?sn?cleos dopamin?rgicos do mesenc?falo. No sentido rostro-caudal, os primeirosneur?nios dopamin?rgicos a surgirem fazem parte da SNc e se estendem at? n?veis maiscaudais do mesenc?falo. A VTA surge nas sec??es rostrais e continua at? o n?vel caudal.No n?vel caudal surge a RRF. A SNc apresentou a exist?ncia de uma subdivis?o, acauda da subst?ncia negra, encontrada em apenas dois outros animais estudados. Opresente estudo indica que os n?cleos dopamin?rgicos do mesenc?falo do Artibeusplanirostris apresenta semelhan?as citoarquitet?nicas, bem como no padr?o dedistribui??o de neur?nios imunorreativos a Tirosina Hidroxilase em compara??o a10 outras esp?cies de mam?feros estudados, com pequenas varia??es, identificada nasubst?ncia negra. / The 3-Hidroxytyramine/dopamine (DA) is a monoamine of catecholamine family and isthe precursor substance synthesis of noradrenaline and adrenaline, having the enzymeTyrosine Hydroxylase (TH) as this regulatory process. In addition, the DA has theability to act as a neurotransmitter in the Central Nervous System - SNC, being themain neurotransmitter of brain nuclei, namely of A8 to A16. The nuclei of the midbrainthat express DA are the Retrorubral Field (RRF, A8), the Substantia Nigra parsCompacta (SNc, A9) and the Ventral Tegmental Area (VTA, A10). Such nuclei areinvolved in complex three circuitry that are the mesostriatal, mesolimbic andmesocotical and are directly related with several behavioral manifestations as motricitycontrol, reward signaling in behavioral learning, motivation and pathologicalconditions, such as Parkinson's Disease and schizophrenia. Interestingly, many of themorphological bases of these neural disturbance remain unknown. Considering therelevance of mesencephalic dopaminergic nuclei, the aim of this research is tocharacterize morphologically the dopaminergic nuclei (clusters A8, A9 and A10) of themidbrain of the bat (Artibeus planirostris). The Artibeus planirostris is a common bat inRio Grande do Norte. Ten animals were used in this research. The animals wereanesthetized, perfused, and the brain was removed from the skull. After dehydration insucrose, the brain was underwent microtomy. Saggital and coronal sections wereobtained and collected in six separate series. The series were Nissl-stained to identifythe cytoarchitectonic boundaries and the other series were subjected toimmunohistochemistry for TH. After cytoarchitectonic analysis and TH+ cellsidentification was possible to establish the anatomical boundaries of the nuclei, as wellas the subdivisions of three of the midbrain dopaminergic nuclei. The SNc is the mostrostral nucleus observed in the midbrain and is identified throughout the rostrocaudalextension of the midbrain. The VTA neurons were seen immediately caudal to the SNcappearance. The RRF neurons were observed just in the caudal levels of the midbrain.The SNc in Artibeus planirostris shows a particular feature, the tail of the SNc. The tailhave been described just in two other studied species. The present work shows aparticular variation in the organizational morphology of the SNc in the artibeus andcontribute to understand the phylogenetic routes by which the dopaminergic system hasevolved.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20254 |
| Date | 26 June 2015 |
| Creators | Medeiros, Helder Henrique Alves de |
| Contributors | 02588073425, http://lattes.cnpq.br/5233940343676740, Ladd, Fernando Vagner Lobo, 86967622153, http://lattes.cnpq.br/0907170560632356, Cavalcanti, Jos? Rodolfo Lopes de Paiva, 04961702447, http://lattes.cnpq.br/3433564869429006, Nascimento J?nior, Expedito Silva do |
| Publisher | Universidade Federal do Rio Grande do Norte, SEM PROGRAMA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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