The use of fossil fuels contributes to global warming and there is a consequent need to
resort to clean and renewable fuels. The major concerns with using agricultural crops
for the production of energy are food and water security. Crops that do not threaten food
security and that can be cultivated with a relatively low amount of water and produce
high yields of fermentable sugars are therefore needed. Sweet sorghum is a fastgrowing
crop that can be harvested twice a year and that can produce both food (grain)
and energy (sugar juice from stems). Sweet sorghum bagasse can also be utilised for
ethanol production.
The aim of this study was to determine the sugar content of different sweet sorghum
cultivars at different harvest times, and determine the cultivar that will produce the
highest ethanol yield at optimized fermentation conditions. Sweet sorghum bagasse was
also pretretated, enzymatic hydrolysed and fermented and the best pretreatment method
and ethanol yield was determined.
In this study, sweet sorghum juice, which mostly consists of readily fermentable sugars
(glucose, sucrose and fructose), as well as the bagasse obtained after juice extraction,
were converted to bio–ethanol. Sweet sorghum juice was fermented to ethanol using
Saccharomyces cereviciae without any prior pretreatment. The effect of pH (4–6), yeast
concentration (1–5g.L–1), initial sugar concentration (110–440g.L–1) and the addition of a
nitrogen source (urea, ammonium sulphate, yeast extract and peptone) on the ethanol
yield was investigated. The pretreatment of bagasse using sulphuric acid (3wt %), and
calcium hydroxide (0.2g/g bagasse), followed by enzymatic hydrolysis using Celluclast
1.5L (0.25g/g bagasse), Novozyme 188 (0.24g/g bagasse) and Tween 80(1.25g.L–1)
were adapted from Mabentsela (2010). Fermentation was done using Saccharomyces
cerevisiae, but it was unable to ferment the xylose sugar.
The results show that the USA 1 cultivar contains the highest sugar content at 3 months.
An ethanol and glycerol yield of 0.48g.g–1 and 0.05g.g–1 was observed respectively at a
pH of 4.5, a yeast concentration of 3wt%, initial sugar concentration of 440g.L–1 and
when ammonium sulphate was added to the fermentation broth as nitrogen source. The glycerol yield formed as a by–product during fermentation and at a maximum ethanol
yield was 0.05g.g–1.
The glucose yield obtained from sulphuric acid, base and ultrasonic wave pretreatment
was 0.79g.g–1, 0.62g.g–1 and 0.62g.g–1 respectively. The glucose yield obtained after
each type of pretreatment was higher than that obtained for unpretreated bagasse, which
was 0.55g.g–1. Base pretreatment, ultrasonic wave pretreatment and unpretreated
bagasse also contained fructose at the end of enzymatic hydrolysis. Base, sulphuric acid
pretreatment disrupted the crystal structure of cellulose and increased the available
surface, and therefore cellulose was easily accessible for enzymatic hydrolysis.
Ultrasonic wave pretreatment showed potential in increasing the surface area for
enzymatic hydrolysis but further investigations need to be done. From bagasse
fermentation, 0.45g.g–1 – 0.39g.g–1 of ethanol per g of available fermentable sugar was
obtained. / Thesis (M.Sc. Engineering Sciences (Chemical Engineering))--North-West University, Potchefstroom Campus, 2012.
| Identifer | oai:union.ndltd.org:NWUBOLOKA1/oai:dspace.nwu.ac.za:10394/7275 |
| Date | January 2012 |
| Creators | Mutepe, Rendani Daphney |
| Publisher | North-West University |
| Source Sets | North-West University |
| Detected Language | English |
| Type | Thesis |
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