Cryopreservation of gametes and embryos is crucial in fertility treatment and fertility preservation. Preservation of oocytes is a more complicated process than preservation of sperm and embryos. According to recent studies, a new preservation technique called vitrification is found to have higher rates of oocytes-survival after warming The aim of this study was to evaluate VitriBlast™ Kit, for vitrification of oocytes. Vitrification is an ultra-rapid freezing method in the presence with of high concentrations of cryoprotectants which avoids intracellular ice-crystal formation during the freezing and warming process. In this study, a total number of 117 immature oocytes were used and 62 of these oocytes were vitrified with VitroBlast™ Kit. During this process two different vitrification devices were used, VitroLoop™ and Cryopette®. After warming, the average survival rate for vitrified oocytes was found to be 61% for VitroLoop™ and 15% for Cryopette® (p<0.001). The remaining 55 oocytes were used as a non-frozen control group and the same incubation method as for vitrified oocytes was used. The survival rate for the control groups was higher than for the vitrified groups (93% versus 35%, p<0.001). The results of this study indicate that VitriBlast™ Kit is not suitable for vitrification of oocytes.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-203471 |
| Date | January 2013 |
| Creators | Gülen Yaldir, Fatma |
| Publisher | Uppsala universitet, Institutionen för kvinnors och barns hälsa |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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