Expressed sequence tags (ESTs) are derived from the developing
tilapia brain was established in our lab. There are 9 transcripts were
identified as thermosensitive/Neural development-related gene. The
effects of different temperatures on the ontogenetic expression of
these thermosensitive/Neural development-related gene during the
critical period of brain sexual differentiation were investigated in the
present study. The ontogenetic expression of inhibitor of DNA
binding/differentiation protein 2 (Id2), thermosensitive/Neural
development-related gene, were enhanced by both lower (20¢J) and
higher (32¢J) temperatures before 10 days post-hatching.
In this study, bioinformatics were searched for Id2, which is a
gene with 738 bp of patial cDNA sequence, open reading frame
(ORF) is 411bp, and deduced 137 amino acids of protein sequence.
The protein of Id2 was expressed in a prokaryotic system, BL21
(Escherichia coli) and purified with Ni-NTA affinity chromatography.
Also, the ORF of Id2 was cloned into pEGFP vector, and
plasmid (pEGFP-Id2) was transfected into the eukaryotic system,
mouse neuroblastoma cell (Neuro-2a cell). The distribution of Id2
expressed in the Neuro-2a cell was identified by fluorescence
microscopy.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0127110-130503 |
| Date | 27 January 2010 |
| Creators | Lu, Yu-nuo |
| Contributors | Juei-Tang Cheng, Ching-Lin Tsai, Li-Hsueh Wang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0127110-130503 |
| Rights | not_available, Copyright information available at source archive |
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