Lau Man Tat. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 95-126). / Abstracts in English and Chinese. / Abstract (in English) --- p.i / Abstract (in Chinese) --- p.iii / Acknowledgements --- p.v / Table of Contents --- p.vi / List of Figures --- p.xi / List of Tables --- p.xiii / Symbols and Abbreviations --- p.xiv / Scientific Names --- p.xvii / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Gonadotropins / Chapter 1.1.1 --- Structure --- p.2 / Chapter 1.1.2 --- Function --- p.6 / Chapter 1.1.3 --- Regulation --- p.9 / Chapter 1.1.3.1 --- Hypothalamic regulators (GnRH) --- p.9 / Chapter 1.1.3.2 --- Endocrine regulators from gonads (steroids) --- p.12 / Chapter 1.1.3.3 --- Paracrine regulators (activin) --- p.14 / Chapter 1.2 --- Activin Family of Growth Factors / Chapter 1.2.1 --- Activin / Chapter 1.2.1.1 --- Structure --- p.14 / Chapter 1.2.1.2 --- Function --- p.14 / Chapter 1.2.1.3 --- Signaling --- p.18 / Chapter 1.2.2 --- Follistatin / Chapter 1.2.2.1 --- Structure --- p.21 / Chapter 1.2.2.2 --- Function --- p.21 / Chapter 1.3 --- Transcriptional regulation of pituitary gonadotropin subunit genes at the promoter level --- p.22 / Chapter 1.4 --- The project objectives and long-term significance --- p.26 / Chapter Chapter 2 --- "Cloning of Smad2, Smad3, Smad4 and Smad7 from the Goldfish Pituitary and Their Involvement in the FSHβ Transcription in LβT2 cells" / Chapter 2.1 --- Introduction --- p.28 / Chapter 2.2 --- Materials and Methods / Chapter 2.2.1 --- Chemicals --- p.31 / Chapter 2.2.2 --- Animals --- p.32 / Chapter 2.2.3 --- Isolation of total RNA --- p.32 / Chapter 2.2.4 --- "Cloning of cDNA fragments of Smad 2, 3, 4 and 7 from the goldfish pituitary" --- p.32 / Chapter 2.2.5 --- Rapid amplification of 5'-cDNA ends (5'-RACE) and full-length cDNA(3'-RACE) --- p.33 / Chapter 2.2.6 --- Primary pituitary cell culture --- p.34 / Chapter 2.2.7 --- "Validation of semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assays for goldfish Smad 2, 3, 4 and7" --- p.35 / Chapter 2.2.8 --- Construction of the reporter plasmid containing the goldfish FSHβ promoter --- p.36 / Chapter 2.2.9 --- Construction of expression plasmids --- p.37 / Chapter 2.2.10 --- Cell culture and transient transfection --- p.38 / Chapter 2.2.11 --- SEAP reporter gene assay --- p.39 / Chapter 2.2.12 --- β-galactosidase reporter gene assay --- p.40 / Chapter 2.2.13 --- Data analysis --- p.40 / Chapter 2.3 --- Results / Chapter 2.3.1 --- "Cloning and sequence characterization of goldfish Smad 2, 3,4 and7" --- p.41 / Chapter 2.3.2 --- "Tissue distribution of Smad 2，3, 4 and 7 expression" --- p.42 / Chapter 2.3.3 --- "Validation of semi-quantitative RT-PCR assays for Smad 2, 3,4 and7" --- p.43 / Chapter 2.3.4 --- Activin regulation of Smad 2，3，4 and 7 expression in cultured goldfish pituitary cells --- p.44 / Chapter 2.3.5 --- "Smad 2, 3 and 7 regulate basal and activin-induced FSHβ transcription in LβT2 cells" --- p.44 / Chapter 2.3.6 --- Autocrine regulation of the gfFSHβ transcription by activin in LβT2 cells --- p.45 / Chapter 2.4. --- Discussion --- p.47 / Chapter Chapter 3 --- Promoter Analysis for the Smad Responsive Element (SRE) in the Goldfish Follicle Stimulating Hormone β(FSHβGene / Chapter 3.1 --- Introduction --- p.71 / Chapter 3.2 --- Materials and Methods / Chapter 3.2.1 --- Chemicals --- p.74 / Chapter 3.2.2 --- Construction of expression plasmids --- p.74 / Chapter 3.2.3 --- Construction of SEAP reporter plasmids containing different lengths of gfFSHβ promoter --- p.74 / Chapter 3.2.4 --- Cell culture and transient transfection --- p.75 / Chapter 3.2.5 --- Reporter gene assays for SEAP and β-Gal --- p.75 / Chapter 3.2.6 --- Data Analyses --- p.76 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Localization of the proximal Smad-responsive Element (SRE) in the gfFHSβ promoter --- p.76 / Chapter 3.4 --- Discussion --- p.78 / Chapter Chapter 4 --- General Discussion / Chapter 4.1 --- Overview --- p.89 / Chapter 4.2 --- Contribution of the present research / Chapter 4.2.1 --- Cloning and characterization of Smad proteins from the goldfish pituitary --- p.90 / Chapter 4.2.2 --- Regulation of Smads in primary pituitary cell culture --- p.90 / Chapter 4.2.3 --- Identification of the Smad responsive element (SRE) on the gfFSHβ promoter --- p.91 / Chapter 4.3 --- Future research direction --- p.93 / References --- p.95
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324451 |
| Date | January 2003 |
| Contributors | Lau, Man Tat., Chinese University of Hong Kong Graduate School. Division of Molecular Biotechnology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xviii, 126 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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