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Dlouhodobé sledování hladin ctDNA u pacientů s metastatickým kolorektálním karcinomem pro včasný záchyt progrese či rekurence onemocnění / Long-term monitoring of ctDNA levels in patients with metastatic colorectal cancer for early detection of progression or recurrence of the disease

Circulating tumor DNA (ctDNA) in peripheral blood of patients with metastatic colorectal cancer appears to be a promising molecular marker that provides various applications. ctDNA levels vary depending on the presence, alternatively on the volume of tumor mass within patient's body, which can be used primarily for early detection of disease progression or recurrence and moreover for evaluating radicality of surgical treatment, all within long-term postoperative follow-up of the patient. Due to minimal invasivity of ctDNA analysis from peripheral blood (so-called liquid biopsy), it is possible to perform it repeatedly at relatively short time intervals. On account of very low fraction of ctDNA in total cell-free DNA (cfDNA) ranging between units and hundreds of percent, the key factor is optimal methodology covering all steps from the isolation process to a sufficiently sensitive detection technology. In this thesis I focus on an optimization of isolation process and analysis of ctDNA obtained from tumor tissue and plasma of selected patients with metastatic colorectal cancer in connection with surgical radicality and correlation with a clinical status of the patients.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:438320
Date January 2021
CreatorsKopalová, Dominika
ContributorsBenešová, Lucie, Kološtová, Katarína
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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