Cardiovascular disease is the leading cause of death in the US, and circulating free fatty acids (FFAs) are known risk factors associated with cardiovascular inflammation. The influence of 14 dietary FFAs (including saturated, mono-, poly-unsaturated and trans), on the expression of inflammatory markers in human coronary arterial smooth muscle (HACSM) and endothelial (HCAEC) cells using a cell culture model was investigated. HACSM and HCAEC cell cultures were incubated with 200 μM of each FFA for 8 or 24 h respectively at 37 °C in a 5% CO2 humidified incubator. Inflammatory biomarkers were assessed by ELISA or Western Blot in the supernatant or cell lysates respectively. Results showed significant differences in the expression of inflammatory biomarkers among the fatty acid treatments and the control, with myristic and palmitic acids being identified as the most and linoleic acid as the least pro-inflammatory. This confirms that FFAs can induce low-grade inflammation in human coronary arterial cells and provides more information on mode of action.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07022013-114311 |
| Date | 07 July 2013 |
| Creators | Soto Rodriguez Gil, Adriana |
| Contributors | Finley, John W., Losso, Jack N., Enright, Frederick M. |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07022013-114311/ |
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