Vibrio parahaemolyticus is a marine bacterium which is the leading cause of bacterial gastroenteritis due to consumption of raw or undercooked bivalve molluscan shellfish in United States and is considered an important seafood-borne pathogen throughout the world. The conventional methods for enumerating this bacteria from seafood is laborious and time consuming, hence the objective of our study was to develop antibody based method which would be sensitive, user-friendly and less time consuming to detect and enumerate Vibrio parahaemolyticus from oysters. In order to condense time and material two rapid and specific antibody based test methods were developed. In the first method, monoclonal antibodies were produced against the polar flagella of Vibrio parahaemolyticus. The monoclonal antibody was employed in Immunomagnetic separation (IMS) protocol and was then coupled with Real-time PCR (q-PCR). IMS coupled to q-PCR was able to detect bacterial numbers in less than 3 hrs with a sensitivity of 1,000 CFU/g of oyster homogenate. In the second method anti-hemolysin serum was produced and employed in Direct colony immunoblot (DCI) protocol to detect pathogenic Vibrio parahaemolyticus. DCI when compared with the currently used conventional method, DNA hybridization (DNAH) showed good correlation and was a faster and simpler method requiring less equipment and stringent laboratory conditions than DNAH. From our results we have found that IMS+q-PCR or DCI could be an useful addition to the methods available for enumerating pathogenic V. parahaemolyticus in seafood.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-04142009-225600 |
| Date | 21 April 2009 |
| Creators | Datta, Shreya |
| Contributors | Witoon Prinyawiwatkul, Paul Wilson, Marlene Janes, Georgianna Tuuri, Beilei Ge |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-04142009-225600/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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