Breast cancer is the leading cause of cancer related mortality in women. The estimated new cases and death for 2010 in the US for females were 207,090 and 39,840 and for men were 1,970 and 390 respectively. Breast cancer cells contain cancer stem cells (CSCs), which represent less than 5% of the total cancer cell population, but accounts for continuous proliferation and growth. Breast cancer is highly metastatic and can spread to various parts of the body. Signaling through cell surface markers, including CD44/CD24/ALDH1 in breast cancer, pro-survival factors, including Hsp90 and other client proteins, angiogenic biomarkers and telomerase activity are essential for cancer cell renewal and could represent putative targets for inhibiting breast cancer cell development. The aim of this research was to determine the in vitro anti-carcinogenic property of ginger oil against breast cancer cells (MDA-MB-231). We investigated the inhibitory activity of ginger oil against the biomarkers that are essential for breast cancer cell viability or proliferation. Cells were incubated with vehicle (DMSO), or ginger oil at concentrations, 0.05% to 0.2% of the volume of the media for 72 h at 37 °C and 5% CO2. Ginger oil dose-dependently inhibited the viability and proliferation of breast cancer cells in vitro. The mechanism of breast cancer cell inhibition included induction of caspase-mediated apoptosis, cell cycle arrest, inhibition of Hsp90 and some of its client proteins, down-regulation of cell surface biomarkers, CD44/ALDH1 in breast cancer cells, inhibition of the biomarkers of angiogenesis, and down-regulation of the activity of telomerase.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07072011-152233 |
| Date | 08 July 2011 |
| Creators | Karki, Namrata |
| Contributors | Losso, Jack, Finley, John, Enright, Frederick |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07072011-152233/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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