Return to search

Vývoj biosenzoru pro fosfatidylinositol / Towards biosensor for phosphatidylinositol

Phosphatidylinositol is a a minor membrane component of eukaryotic cells, however, it plays a crucial role in cell signaling pathways as a precursor for a number of signaling molecules and second messengers. Among the most significant ones are phosphoinositides created by phosphorylation of the hydroxyl groups of phosphatidylinositol at positions 3,4, and 5 of the inositol ring. Despite its significance, the spatial and temporal distribution and dynamics of phosphatidylinositol remains unclear owing mainly to the lack of a specific optical probe (biosensor) to visualize phosphatidylinositol in living cells. Biosensor for inositol phospholipids are based on lipid-binding domains of their effector proteins with high enough affinity and specificity for a given phosphoinositide - but nosuch domain is known for PI. However, an enzyme - phosphatidylinositol-dependent phospholipase C - that specifically recognizes phosphatidylinositol is known. This enzyme catalyzes the hydrolysis of phosphatidylinositol into diacylglycerol and inositol 1-phosphate and unlike eukaryotic homologs does not act upon the phosphorylated forms of phosphatidylinositol. The main aim of this thesis was to solve the structures of several inactive mutant forms of phospholipase C from Bacillus cereus complexed to myo-inositol which...

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:365118
Date January 2017
CreatorsEisenreichová, Andrea
ContributorsBouřa, Evžen, Petráčková, Denisa
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

Page generated in 0.0016 seconds