Light is one of the main environmental factors largely determining plant life. Light provides energy resources for photosynthesis and it is also the most important medium of perception as it represents the main source of information about plant surroundings. My diploma thesis entitled “Light and its effect on plant proteome” briefly summarizes present knowledge about the light perception in plants, its interpretation of light quality, quantity, and direction via photoreceptors. These components trigger a whole range of changes, both on molecular and developmental levels, but the complex molecular mechanisms regulating system of light signalling in plants are far from being resolved. Here, I followed effects of different light parameters on plant proteome, namely, diurnal variations, low- and high-light intensity, spectral composition, and a change in the light period duration. To study these effects two model organisms were selected (i) Arabidopsis thaliana as a representative plant model with the most annotated genome/proteome and (ii) Solanum lycopersicum as a representative plant model significant for agriculture and biotechnology. Proteome changes in response to different light factors were analyzed by LC-MS. In total, the analyses revealed over 100 and 300 light-responsive proteins in Arabidopsis and S. lycopersicum, respectively. Proteomics provided highly sensitive snapshots of protein abundance in response to light and selected experiments have been complemented with a GC-MS metabolite profiling, conductivity measurements, and fluorescence microscopy.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:426996 |
| Date | January 2018 |
| Creators | Luklová, Markéta |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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