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Previous issue date: 2017-11-27 / A exposi??o ? fatores ambientais, n?veis elevados de xenobi?ticos na dieta e at? as
rea??es bioqu?micas realizadas pelo metabolismo humano aumentam a gera??o de
esp?cies reativas de oxig?nio (ERO) e nitrog?nio (ERN). Essa eleva??o de ERO e
ERN pode promover estresse oxidativo e gerar uma s?rie de problemas
fisiopatol?gicos. Estudos bioqu?micos relatam que os antioxidantes diet?ticos
possuem benef?cios na preven??o de doen?as. As frutas se destacam pela grande
quantidade de subst?ncias bioativas com essa atividade ben?fica. A atem?ia, nosso
objeto de estudo, ainda foi pouco estudada e portanto n?o se sabe seus
componentes qu?micos (nutrientes) e seus efeitos no metabolismo ap?s sua ingesta.
Portanto, ? muito importante compreender os mecanismos da rea??o dos
antioxidantes presentes na fruta atem?ia com as esp?cies reativas. Neste trabalho
foram feitos ensaios in vitro e in vivo, dos quais destacam-se a forma??o de extrato
aquoso e farinha das partes da semente, casca e polpa, quantifica??o de prote?nas,
carboidratos e compostos fen?licos, como tamb?m a avalia??o da presen?a de
inibidores de proteases e hemaglutininas, al?m dos testes antioxidantes; capacidade
antioxidante total (CAT), sequestro de radicais hidroxila (SRH), sequestro de radicais
super?xido (SRS), quela??o de metais (QM), al?m da avalia??o de estresse
oxidativo por par?metros bioqu?micos e histol?gico em animais alimentados com as
partes da atemoia. Foram detectados a presen?a de inibidores de protease e
aglutininas nos extratos da atemoia, essas subst?ncias possuem potencial para
serem utilizadas na ?rea da biotecnologia ou biom?dica, ajudando na preven??o de
doen?as ou at? mesmo no melhoramento gen?tico das frutas. Os extratos
apresentaram prote?nas, carboidratos e compostos fen?licos em sua composi??o,
fato que sugere uma sinergia entre esses compostos no efeito antioxidante, vale
ressaltar que no estudo sup?em-se a presen?a de outras subst?ncias n?o
conhecidas, que podem estar influenciando nesses efeitos. J? os estudos in vivo
mostram um efeito n?o t?xico dos extratos. Os melhores resultados in vivo foram
obtidos com extrato semente. Sendo assim, prop?em-se que os extratos de atemoia
tem propriedades funcionais. E estudos futuros devem ser realizados com intuito de
se elucidar as vias e compostos espec?ficos que possam atuar nesses benef?cios. / An exhibition of human food, biochemistry and mathematics, increase the generation
of reactive oxygen species (ROS) and nitrogen (RN). This elevation of ROS and RNS
creates an oxidative stress, which can generate a series of pathophysiological
problems. Biochemical studies report that dietary antioxidants have benefits in
disease prevention. Fruits stand out for the great amount of bioactive substances
with this beneficial activity. Nutrition is one of them, however, it has been little studied
and it is not possible to know its chemical components (nutrients) and their effects on
metabolism after its ingestion. Therefore, it is very important for the mechanisms of
the reaction of the antioxidants present in the fruit to the redo with the reactive
species. In this work, in vitro and in vivo assays, which include the formation of
aqueous extract and flour of the parts of the seed, peel and pulp, quantification of
proteins, carbohydrates and phenolic compounds, as well as an evaluation of the
presence of protease inhibitors and hemagglutinins, in addition to the antioxidant
testes; total antioxidant capacity (CAT), hydroxyl radical sequestration (SRH),
superoxide radical scavenging (SRS), metal chelation (QM), and biochemical and
histological evaluation of oxidative stress in animals fed as parts of atemoia. A
presence of protease inhibitors and agglutinins have been detected in atemoia
extracts, these are necessary for use in the biotechnology or biomedical area,
helping to prevent diseases or even genetic improvement of fruits. Extruders
presented proteins, carbohydrates and phenolic compounds in their composition, a
fact that suggests a synergy between these compounds, it is not an antioxidant
effect, it is worth mentioning that the study supposes a presence of other substances
not known, which may be influencing these effects . Already in vivo studies show a
nontoxic effect of the extracts. The best in vivo results were obtained with seed
extract. Thus, it is proposed that the extracts of atemoia have properties. Future
studies should be carried out to become segmental and specific segments.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24782 |
| Date | 27 November 2017 |
| Creators | Serquiz, Alexandre Coelho |
| Contributors | 76111830449, Uchoa, Adriana Ferreira, 58024867320, Giordani, Raquel Brandt, 00104164042, Maciel, Maria Aparecida Medeiros, 37320165449, Fernandes, Thales Allyrio Ara?jo de Medeiros, 02751517439, Rocha, Hugo Alexandre de Oliveira |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS DA SA?DE, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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