This thesis deals with description and verification of different methods of DNA isolation from insect tissue of order Lepidoptera -- butterflies with an emphasis on family Sphingidae -- hawk moths and subsequent amplification of COI gene by PCR method. All tested methods were compared and evaluated in terms of efficiency, yield and purity of the DNA isolation, duration and financial costs. We selected to establish a methodology which is sufficient for obtaining COI gene sequences using genetic analyzer, its application in the system BoldSystems and subsequent determination of the sample. This is important in effort to map the species diversity.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:250441 |
| Date | January 2015 |
| Creators | Novák, Vojtěch |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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