Acute lymphoblastic leukemia (ALL) is the most common malignancy occurring in childhood and accounts for 77% of all leukemia cases. Long-term survival is greater than 80% with appropriate treatment. Chemotherapy is the most widely used treatment, but it can have significant side effects including neutropenia and immunosuppression.
Interleukin (IL-10) is an immunoregulatory cytokine with anti-inflammatory effects. It inhibits some cells like macrophages while stimulating other cells like B cells. IL-10 has been found to be involved in conditions involving an immune response and inflammation, including pneumonia, septic shock, and graft versus host disease (GVHD).
Myeloperoxidase (MPO), which catalyzes the production of hypochlorite from chlorides and hydrogen peroxide, is an abundantly expressed hemoprotein with anti-microbial effects and a major player in host defense. MPO has been implicated in the pathogenesis of a number of conditions and associated with certain infections.
In this study, polymorphisms in MPO and IL-10 were investigated to test their association with risk of infection in a population of Caucasian patients with ALL. Genotyping was performed by enzyme digest for IL-10 polymorphism -592(C/A) and by pyrosequencing for the MPO polymorphisms -129(G/A) and -463(G/A). Allele frequencies at each site were in Hardy Weinberg Equilibrium (HWE). No significant correlation was found between the genotype at IL-10 -592, MPO -129 or MPO -643 and infection episodes in our patient population. As the study population was relatively small, no strong conclusions can be drawn, but implications for further research can be identified.
Public Health Significance: Knowledge of the effects of certain genetic polymorphisms may be important when treating patients with ALL. Patients at increased risk of infection may require prophylaxis or more intense surveillance to ensure a better outcome.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-04142005-184746 |
| Date | 27 September 2005 |
| Creators | Burans, Courtney Rachael |
| Contributors | Rakesh Goyal, MD, John Wilson, PhD, Robert Ferrell, PhD |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-04142005-184746/ |
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