The non-phosphorylated oxidative pathway of glucose dissimilation has been confirmed in Pseudomonas aeruginosa using whole cells and cell-free extracts. The oxidation of glucose to 2-ketogluconate was complete and stoichiometric in cell-free extracts and cell-free extracts of glucose grown cells were shown to be incapable of metabolizing 2-ketogluconate.
It was shown that whole cells completely degraded 2-ketogluconate and quantitatively accumulated pyruvic acid in the presence of specific inhibitors. The initial step involved in 2-ketogluconate dissimilation was found to be exceptionally labile to the effects of a variety of metabolic inhibitors.
The metabolism of 2-ketogluconate was demonstrated to involve the initial phosphorylation with adenosine triphosphate
(ATP) as the phosphate donor. The resultant intermediate, 2-keto-6-phosphogluconate, was identified and was shown to undergo reduction by a nicotinamide adenine dinucleotide phosphate linked reductase to 6-phosphogluconate which, in turn, was metabolized to pyruvate by enzymes of the Entner-Doudoroff pathway.
Radioactivity from 2-ketogluconate-C¹⁴ was rapidly incorporated into cellular constituents, primarily protein, by washed cell suspensions of P. aeruginosa, but oxidation
of 2-ketogluconate did not involve the accumulation of keto-acid intermediates.
The role of 2-ketogluconic acid as a key intermediate
for the conservation of excess carbon under conditions where nitrogen is limiting was discussed. / Land and Food Systems, Faculty of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/37075 |
| Date | January 1965 |
| Creators | Kay, William Wayne |
| Publisher | University of British Columbia |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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