<p>The methylotrophic yeast, <i>Pichia pastoris </i>(recently reclassified as <i>spp. Komagatella</i>) has long been regarded as a useful host organism
for the production of recombinant proteins, particularly when using the AOX
system which utilizes methanol as both the inducing agent as well as the
primary carbon source for growth and energy. Significant historical work has
shown that growth rate and protein productivity can be correlated to methanol
concentration. However, the relationship between oxygen and protein
productivity are less consistent. While with many variations models having been
developed and used for analyzing culture kinetics, these models have only been
applied to methanol concentration. Furthermore, while results for methanol are
fairly consistent, oxygen considerations have been far less consistent. </p>
<p> </p>
<p>This work presents various bi-variate models which
includes considerations for growth and inhibition for both methanol and oxygen
with this expanded model showing strong alignment to previous works to both
oxygen and methanol data. While more work is necessary to fully confirm and
validate which form of the bivariate model is most appropriate, this work
provides a framework necessary to expand analysis to include oxygen
considerations. This framework has the potential to be used to further inform
selection of feeding methodology as well as direct investigations into
metabolic studies. </p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/8309597 |
| Date | 13 August 2019 |
| Creators | Robert Michael Binkley (6867047) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/BI-VARIATE_GROWTH_MODEL_OF_PICHIA_PASTORIS_INCLUDING_OXYGEN_CONSIDERATIONS_AND_ITS_IMPORTANCE_IN_RECOMBINANT_PROTEIN_PRODUCTION/8309597 |
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