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Properties of Normal Rat Kidney Cells Transformed by a Temperature-Sensitive Mutant (LA31) of Rous Sarcoma Virus

The basis of this investigation is to characterize growth property differences in normal versus virally transformed cells. Using a temperature-sensitive mutant of Rous sarcoma virus, the cells' transformation state is regulated by the growth temperature; at 33°C the cells are transformed, while at 39°C the cells have normal characteristics. The morphology of NRK cells is elongated and fibroblastic; when transformed the cells are rounded. Normal cells grow to a monolayer and stop, while transformed cells grow to saturation densities greater than just a monolayer amount. Transformed cells can form foci when grown in mixture with normal cells. Normal cells must be in contact with the culture vessel in order to grow, but transformed cells lack anchorage dependence for growth.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc503838
Date08 1900
CreatorsConnolly, John R. (John Robert)
ContributorsChen, Young C., Johansson, Karl R., Zimmerman, Earl G.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatv, 39 leaves : ill., Text
RightsPublic, Connolly, John R. (John Robert), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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