The histone 2A (H2A) variant Z (H2A.Z) regulates gene expression, replacing H2A predominately at +1 nucleosomes. Whereas, H2A.Z can act as either a positive or negative transcriptional regulator, this research focuses on its transcriptional activation role. This thesis focuses on examining the Glycine max HTA9 gene family (H2A.Z9). The Arabidopsis thaliana HTA9 protein sequence was used to query the G. max proteome resulting in identification of five H2A.Z9 (H2A.Z9-1-5) paralogs. H2A.Z9-1, H2A.Z9-2, H2A.Z9-4, and H2A.Z9-5 are expressed within Heterodera glycines-parasitized root cells undergoing defense. All 5 paralogs, including H2A.Z9-3, were studied in transgenic-functional analyses. Data demonstrates that H2A.Z9 overexpression leads to a 60-70% reduction in H. glycines- parasitism with no effect on root growth. In contrast, H2A.Z9 knockdown by RNAi results in a 3.5-5.0-fold increase in H. glycines-parasitism with no effect on root growth. These results demonstrate that H2A.Z9 is important to G. max defense toward H. glycines-parasitism and indicate possible redundant or specific roles for each paralog.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-6073 |
| Date | 30 April 2021 |
| Creators | Acharya, Sudha |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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