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Demographic and molecular indicators of environmental stresses in Moina macrocopa and Daphnia magna.

Ho, Sin Chu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 112-135). / Abstracts in English and Chinese. / Abstract --- p.i / 摘要 --- p.ii / Acknowledgement --- p.iii / Table of Contents --- p.iv / List of Abbreviations --- p.vii / List of Figures --- p.x / List of Tables --- p.xii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Test Organisms: Daphnia magna and Moina macrocopa --- p.1 / Chapter 1.2 --- Biomarkers --- p.2 / Chapter 1.3 --- Heat shock protein (HSP) --- p.4 / Chapter 1.3.1 --- Regulation of hsp70 gene expression --- p.5 / Chapter 1.3.2 --- Review of studies on hsp70 of various species --- p.6 / Chapter 1.3.3 --- Review of studies using molecular methods in Daphnia and Moina --- p.7 / Chapter 1.4 --- Environmental stress studied --- p.9 / Chapter 1.5 --- Objectives --- p.11 / Chapter Chapter 2 --- Materials and Methods --- p.12 / Chapter 2.1 --- Experimental organisms --- p.12 / Chapter 2.2 --- "The effects of temperature, metals and nutritional stress on cladocerans" --- p.13 / Chapter 2.3 --- Demographic measurement --- p.15 / Chapter 2.4 --- Cloning and sequencing of D. magna and M. macrocopa hsp70 --- p.17 / Chapter 2.4.1 --- Acute heat shock --- p.17 / Chapter 2.4.2 --- Total RNA extraction --- p.17 / Chapter 2.4.3 --- Reverse transcription and polymerase chain reaction (PCR) --- p.18 / Chapter 2.4.4 --- Degenerative PCR --- p.21 / Chapter 2.4.5 --- Cloning and sequencing of hsp70 partial sequence --- p.25 / Chapter 2.4.6 --- Rapid amplification of cDNA ends (RACE) --- p.28 / Chapter 2.4.7 --- Confirmation of D. magna and M. macrocopa hsp70 sequences --- p.30 / Chapter 2.5 --- Molecular measurements - heat shock protein 70 gene expression --- p.31 / Chapter 2.5.1 --- Sample collection --- p.31 / Chapter 2.5.2 --- Quantification of cDNA level by real-time PCR --- p.32 / Chapter 2.5.2.1 --- Primer design --- p.32 / Chapter 2.5.2.2 --- Validation of real-time PCR conditions --- p.32 / Chapter 2.5.2.3 --- Determination of hsp70 gene expression levels --- p.35 / Chapter 2.6 --- Statistical analysis --- p.36 / Chapter Chapter 3 --- Results --- p.37 / Chapter 3.1 --- Demographic measurements --- p.37 / Chapter 3.1.1 --- Effects of temperature on life history parameters of M macrocopa --- p.37 / Chapter 3.1.2 --- Effects of temperature on life history parameters of D. magna --- p.40 / Chapter 3.1.3 --- Effects of Metals on life history parameters of M. macrocopa --- p.42 / Chapter 3.1.4 --- Effects of Anabaena on life history parameters of M. macrocopa --- p.47 / Chapter 3.2 --- Cloning and sequencing of hsp70 --- p.50 / Chapter 3.2.1 --- Partial sequences of D. magna and M. macrocopa hsp70 --- p.50 / Chapter 3.2.2 --- Sequences of 3´ة and 5´ة RACE amplified products --- p.53 / Chapter 3.2.3 --- Full sequences of D. magna and M. macrocopa hsp70 --- p.53 / Chapter 3.2.4 --- Phylogenetic relationships of the hsp70 gene --- p.60 / Chapter 3.3 --- Molecular measurements - heat shock protein 70 gene expression --- p.71 / Chapter 3.3.1 --- Validation of real-time PCR conditions --- p.71 / Chapter 3.3.2 --- Effect of temperature stress on hsp70 gene expression levels --- p.76 / Chapter 3.3.3 --- Effect of metals on hsp70 gene expression levels --- p.78 / Chapter 3.3.4 --- Effect of Anabaena on Hsp70 Gene Expression Levels --- p.83 / Chapter Chapter 4 --- Discussion --- p.85 / Chapter 4.1 --- Demographic responses of M. macrocopa and D. magna by different stress --- p.85 / Chapter 4.1.1 --- Effects of temperature on life history parameters --- p.85 / Chapter 4.1.2 --- Effects of metal stress on life history parameters --- p.87 / Chapter 4.1.3 --- Effects of Anabaena on life history parameters --- p.89 / Chapter 4.2 --- Characterization of hsp70 of M. macrocopa and D. magna --- p.92 / Chapter 4.3 --- Hsp70 gene expression in response to different stress --- p.94 / Chapter 4.3.1 --- Effect of temperature stress on hsp70 gene expression levels --- p.94 / Chapter 4.3.2 --- Effect of metals on Hsp70 gene expression levels --- p.97 / Chapter 4.3.3 --- Effect of Anabaena on hsp70 gene expression levels --- p.101 / Chapter 4.4 --- Comparison of demographic and molecular measurements --- p.102 / Chapter 4.5 --- Performance of hsp70 gene expression as a biomarker of environmental stress --- p.106 / Chapter 4.6 --- Conclusion --- p.111 / References --- p.112 / Appendix --- p.136

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326497
Date January 2008
ContributorsHo, Sin Chu., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiii, 139 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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