Return to search

Recognition of Chinese medicinal herbs by gas chromatgraphy [sic]. / Recognition of Chinese medicinal herbs by gas chromatography

by Suk Che Ho. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 86-88). / Abstract also in Chinese. / Abstract --- p.i / Acknowledgments --- p.iii / Dedication --- p.iv / Abbreviations --- p.v / Table of Contents --- p.vi / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Overview of Chinese Medicinal Herbs containing essential oils --- p.1 / Chapter 1.1.1 --- Introduction of Chinese Medicinal Herbs --- p.1 / Chapter 1.1.2 --- Chinese Medicinal Herbs containing essential oils --- p.2 / Chapter 1.2 --- Recognition of Chinese Medicinal Herbs --- p.2 / Chapter 1.2.1 --- Traditional method in recognition of Chinese Medicinal Herbs (CMH) --- p.2 / Chapter 1.2.2 --- Instrumental Methods for the recognition of CMH --- p.4 / Chapter 1.2.3 --- The use of GC and GC/MS on CMH --- p.4 / Chapter 1.3 --- Motivation and objective of this research --- p.5 / Chapter 1.3.1 --- Motivation --- p.6 / Chapter 1.3.2 --- Objective of this research --- p.7 / Chapter 1.4 --- Outline of the methodology and arrangement of the thesis --- p.8 / Chapter Chapter 2: --- Experimental Setup --- p.11 / Chapter 2.1 --- Reagents and materials --- p.11 / Chapter 2.1.1 --- Reagents and glassware --- p.11 / Chapter 2.1.2 --- Materials --- p.11 / Chapter 2.2 --- Sample pretreatment --- p.14 / Chapter 2.3 --- Extraction of essential oils from the herbal samples --- p.14 / Chapter 2.3.1 --- Traditional extraction methods for essential oils --- p.14 / Chapter 2.3.2 --- Extraction by hydrodistillation using Dean and Stark-type trap --- p.15 / Chapter 2.4 --- Results --- p.17 / Chapter 2.4.1 --- Comparison with literature data --- p.17 / Chapter 2.4.2 --- Reproducibility of the extraction --- p.17 / Chapter 2.4.3 --- Recovery test --- p.26 / Chapter 2.5 --- Discussion --- p.27 / Chapter Chapter3: --- Instrumental Analysis of the Essential Oils --- p.29 / Chapter 3.1 --- GC analysis --- p.29 / Chapter 3.1.1 --- Instrumentation --- p.29 / Chapter 3.1.2 --- Instrumental settings --- p.31 / Chapter 3.1.3 --- The use of GC in the analysis of essential oils --- p.31 / Chapter 3.1.3.1 --- Qualitative data --- p.31 / Chapter 3.1.3.2 --- Quantitative data --- p.33 / Chapter 3.1.3.3 --- Dilution strategy --- p.33 / Chapter 3.1.4 --- Results --- p.36 / Chapter 3.1.4.1 --- Precision test --- p.36 / Chapter 3.1.4.2 --- Linearity --- p.39 / Chapter 3.2 --- GC/MS analysis --- p.41 / Chapter 3.2.1 --- Instrumentation --- p.41 / Chapter 3.2.2 --- Instrumental settings --- p.42 / Chapter 3.2.3 --- The use of GC/MS in the analysis of essential oils --- p.43 / Chapter 3.2.3.1 --- Identification by GC/MS --- p.43 / Chapter 3.2.3.2 --- Abundance information --- p.43 / Chapter 3.2.4 --- Results --- p.44 / Chapter 3.2.4.1 --- Precision test --- p.44 / Chapter 3.2.4.2 --- Linearity --- p.46 / Chapter 3.2.4.3 --- Detection limit --- p.48 / Chapter 3.2.4.4 --- Chromatographic patterns of herbal samples obtained by GC/MS --- p.49 / Chapter 3.3 --- Discussion --- p.49 / Chapter Chapter 4: --- Development of a system for recognition --- p.52 / Chapter 4.1 --- Introduction --- p.52 / Chapter 4.2 --- Analysis of chromatographic patterns --- p.53 / Chapter 4.2.1 --- Extraction of “effective´ح peaks --- p.54 / Chapter 4.2.2 --- Extraction of “characteristic´ح peaks --- p.56 / Chapter 4.3 --- Library section --- p.60 / Chapter 4.3.1 --- Calculation of relative retention indices --- p.60 / Chapter 4.3.2 --- Normalization factors --- p.61 / Chapter 4.4 --- Matching section --- p.62 / Chapter 4.4.1 --- Overview of the matching method --- p.62 / Chapter 4.4.2 --- Input --- p.63 / Chapter 4.4.3 --- Matching strategy --- p.64 / Chapter 4.4.4 --- Matching algorithms --- p.64 / Chapter 4.4.4.1 --- "Matching with “characteristic"" peaks" --- p.64 / Chapter 4.4.4.2 --- Matching with “effective´ح peaks --- p.65 / Chapter 4.4.5 --- Calculation of similarity scores --- p.66 / Chapter 4.4.6 --- Output --- p.69 / Chapter Chapter 5: --- Performance of the proposed recognition system --- p.70 / Chapter 5.1 --- Recognition performance of the database --- p.70 / Chapter 5.1.1 --- Definition of similarity --- p.70 / Chapter 5.1.2 --- Performance test of the recognition method --- p.70 / Chapter 5.1.2.1 --- Candidates in the library file --- p.70 / Chapter 5.1.2.2 --- Unknown not found in the library file --- p.75 / Chapter 5.1.3 --- Information drawn from the scores --- p.77 / Chapter 5.1.3.1 --- Recognition of the unknown sample in terms of similarity --- p.77 / Chapter 5.1.3.2 --- Relationship between the herbal drugs --- p.79 / Chapter 5.2 --- Applicability of the proposed methodology --- p.80 / Chapter 5.3 --- Limitation of the proposed methodology --- p.81 / Chapter 5.4 --- Future prospect --- p.81 / Chapter Chapter 6: --- Conclusion --- p.83 / References --- p.86 / Appendices / Chapter A. --- Linearity of calibration graphs using GC --- p.A1 / Chapter B. --- Linearity of calibration graphs using GC/MS --- p.A3 / Chapter C. --- GC/MS chromatograms of the herbal samples --- p.A5 / Chapter D. --- "Relative retention times of “effective"" and ""characteristic"" peaks" --- p.A28

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322415
Date January 1998
ContributorsHo, Suk Che., Chinese University of Hong Kong Graduate School. Division of Chemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, ix, 88, 34 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Page generated in 0.0022 seconds