Nine distinctive bacteria were isolated from a diclofop-methyl-degrading-consortium biofilm and their genomic DNA extracted for genetic characterization. With one exception, all the isolates contained plasmid(s). The consortium degrades diclofopmethyl producing a number of metabolites that are intermediates or substrates for bacteria that degrade chlorinated aromatic compounds. Accordingly, specific catabolic gene PCR primers for chlorinated degradation pathways were designed and tested to determine if these genes are involved in diclofop-methyl degradation. DNA homology analysis between the PCR products and the known catabolic genes investigated by Southern hybridization analysis and by sequencing, suggested that novel catabolic genes are functioning in the isolates. Specific fluorescent oligonucleotides were designed for two of the isolates following the 16S rDNA sequencing and the identification of each of the isolates. These probes were successfully used in whole cell hybridization and will be used in subsequent studies of the microbial ecology of the consortium.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27359 |
| Date | January 1997 |
| Creators | Laramée, Louise. |
| Contributors | Greer, C. W. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001574731, proquestno: MQ29733, Theses scanned by UMI/ProQuest. |
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